Diabetic conditions downregulate the expression of CD2AP in podocytes via PI3-K/Akt signalling

Title
Diabetic conditions downregulate the expression of CD2AP in podocytes via PI3-K/Akt signalling
Author(s)
한기동홍은정[홍은정]하태선[하태선]
Keywords
LACKING CD2-ASSOCIATED PROTEIN; SLIT DIAPHRAGM; NEPHROTIC SYNDROME; CELL CONTACTS; NEPHRIN; 3-KINASE; KINASE; CYTOSKELETON; SURVIVAL; P130CAS
Issue Date
201501
Publisher
WILEY-BLACKWELL
Citation
DIABETES-METABOLISM RESEARCH AND REVIEWS, v.31, no.1, pp.50 - 60
Abstract
BackgroundProteinuria is typically accompanied by structural and compositional changes of the foot processes and of the slit diaphragms between podocytes. CD2-associated protein (CD2AP) in podocytes serves as an adaptor protein binding to nephrin and podocin, anchoring these slit diaphragm proteins to actin filaments of podocyte cytoskeleton and sending signals inward or outward. MethodsIn the present study, we prepared streptozotocin-induced diabetic renal tissues and cultured podocytes in diabetic conditions to investigate podocyte phenotypical changes, including quantitative and distributional changes of CD2AP protein and search for the signalling mechanisms in diabetic conditions. We prepared cultured rat glomerular epithelial cells and mouse podocytes to study how high glucose and advanced glycosylation end products (AGE) induce phenotypical changes of cultured podocyte, under (1) normal glucose (5mM, = control), (2) high glucose (30mM), (3) AGE-added or (4) high glucose plus AGE-added conditions. ResultsAccording to diabetic duration, density of CD2AP in renal tissue of experimental diabetic nephropathy became conglomerulated and diminished. In cultured podocytes, CD2AP co-localized with nephrin and zonula occludens-1 by confocal imaging. High glucose and high glucose plus AGE induced the relocalization and concentration of CD2AP at internal cytoplasmic and perinuclear areas of podocytes. High glucose plus AGE-added condition also decreased CD2AP protein amount and its mRNA expression compared with normal glucose or osmotic control conditions. In addition, LY294002, a phosphoinositide 3-kinase inhibitor, prevented the quantitative and distributional changes of CD2AP induced by high glucose and AGE. ConclusionsThese findings suggest that diabetic conditions induce the phenotypical changes of podocyte CD2AP possibly via phosphoinositide 3-kinase/Akt signalling. Copyright (c) 2014 John Wiley & Sons, Ltd.
URI
http://hdl.handle.net/YU.REPOSITORY/33755http://dx.doi.org/10.1002/dmrr.2562
ISSN
1520-7560
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자연자원대학 > 식품공학과 > Articles
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