Crystallization and preliminary X-ray crystallographic analysis of the CARD domain of apoptosis repressor with CARD (ARC)
- Crystallization and preliminary X-ray crystallographic analysis of the CARD domain of apoptosis repressor with CARD (ARC)
- 박현호; 김성현
- CASPASE RECRUITMENT DOMAIN; CELL-DEATH; PATHWAYS; CANCER
- Issue Date
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.71, pp.82 - 85
- Apoptosis repressor with caspase-recruiting domain (ARC) is an apoptosis repressor that inhibits both intrinsic and extrinsic apoptosis signalling. Human ARC contains an N-terminal caspase-recruiting domain (CARD domain) and a C-terminal proline- and glutamic acid-rich (P/E-rich) domain. The CARD domain in ARC is the domain that is directly involved in inhibition of the extrinsic pathway. In this study, the N-terminal CARD domain of ARC was overexpressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.1 angstrom and the crystals were found to belong to space group P6(1) or P6(5), with unit-cell parameters a = 98.28, b = 98.28, c = 51.86 angstrom, alpha= 90, beta = 90, gamma = 120 degrees.
- Appears in Collections:
- 이과대학 > 화학생화학부 > Articles
생명공학부 > 생명공학부 > Articles
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)