Measurement of Antibodies to Varicella-Zoster Virus Using a Virus-Free Fluorescent-Antibody-to-Membrane-Antigen (FAMA) Test

Title
Measurement of Antibodies to Varicella-Zoster Virus Using a Virus-Free Fluorescent-Antibody-to-Membrane-Antigen (FAMA) Test
Author(s)
박호선황지영박락현[박락현]이강일[이강일]남궁심[남궁심]최석근[최석근]박송용[박송용]박준수[박준수]
Keywords
LINKED-IMMUNOSORBENT-ASSAY; GLYCOPROTEIN ANTIGEN; IMMUNITY; VACCINE; COMPLEX; CELLS; GL; GH
Issue Date
201502
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.2, pp.268 - 273
Abstract
The fluorescent-antibody-to-membrane-antigen (FAMA) test is regarded as the "gold standard" to detect protective antibodies to varicella-zoster virus (VZV) because of its high sensitivity and specificity. Because the classic FAMA test uses an infectious virus for detection of antibodies to VZV, it is labor-intensive, and also requires special equipment for handling the virus. For this reason, we attempted to develop a simple and safe FAMA assay. Because VZV glycoprotein E (gE) is one of the major VZV glycoproteins, we used the gE protein for the FAMA test (gE FAMA). Here, we demonstrate that overexpression of gE in HEK293T cells can be used to measure antibodies in human serum, and that gE FAMA titers are closely correlated with gpEIA ELISA data. These results indicate that our gE FAMA test has the potential to measure antibodies to VZV.
URI
http://hdl.handle.net/YU.REPOSITORY/33554http://dx.doi.org/10.4014/jmb.1408.08048
ISSN
1017-7825
Appears in Collections:
의과대학 > 미생물학교실 > Articles
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