Human adipose-derived stem cells attenuate inflammatory bowel disease in IL-10 knockout mice

Title
Human adipose-derived stem cells attenuate inflammatory bowel disease in IL-10 knockout mice
Author(s)
송인환정우연강주환[강주환]김경곤김희선장병익박용훈
Keywords
CHRONIC COLITIS; CROHNS-DISEASE; ANIMAL-MODELS; T-CELLS; THERAPY; REGENERATION; HOMEOSTASIS; INFLIXIMAB; INTESTINE; BONE
Issue Date
201502
Publisher
CHURCHILL LIVINGSTONE
Citation
TISSUE & CELL, v.47, no.1, pp.86 - 93
Abstract
Inflammatory bowel disease (IBD) is a complex immunological disorder characterized by chronic inflammation caused mainly by unknown factors. The interleukin-10 knockout (IL-10 KO) mouse is a well established murine model of IBD which develops spontaneous intestinal inflammation that resembles Crohn's disease. In the present study, human adipose-derived mesenchymal stem cells (hAMSCs) were administrated to IL-10 KO mice to evaluate the anti-inflammatory effects of hAMSCs that may attenuate the progress of or treat IBD. After IBD was induced by feeding the IL-10 KO mouse a 125-250 ppm piroxicam mixed diet for 1 week, 2 x 10(6) hAMSCs were injected into the peritoneum and the mice were switched to a normal diet. After 1 week, the mice were sacrificed and tissue samples were harvested. Tissue scores for inflammation and inflammation-related genes expression were determined. The hAMSC-treated group showed significantly reduced inflammatory changes in histological analysis. Reverse transcription-PCR analysis showed that RANTES, Toll-like receptor 9, and IL-4 expression levels were not significantly different between the groups while IL-12, INF-gamma, and TNF-alpha levels were significantly decreased in the hAMSC treated group. hAMSC attenuated IBD in the IL-10 KO mice by suppressing inflammatory cytokine expression, was mediated by the type 1 helper T cell pathway. Even though only a single injection of hAMSCs was delivered, the effect influenced chronic events associated with inflammatory changes and demonstrated that hAMSCs are a powerful candidate for IBD therapy. (C) 2014 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/33500http://dx.doi.org/10.1016/j.tice.2014.12.001
ISSN
0040-8166
Appears in Collections:
의과대학 > 해부학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
의과대학 > 미생물학교실 > Articles
의과대학 > 내과학교실 > Articles
의과대학 > 소아청소년과학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE