Specific Discrimination of Three Pathogenic Salmonella enterica subsp enterica Serotypes by carB-Based Oligonucleotide Microarray

Title
Specific Discrimination of Three Pathogenic Salmonella enterica subsp enterica Serotypes by carB-Based Oligonucleotide Microarray
Author(s)
서정현신화희[신화희]황병희[황병희]차형준[차형준]
Keywords
CARBAMOYL-PHOSPHATE SYNTHETASE; 16S RIBOSOMAL-RNA; DNA MICROARRAY; BACTERIAL PATHOGENS; SECONDARY STRUCTURE; FOODBORNE OUTBREAK; MULTIPLE DETECTION; ESCHERICHIA-COLI; UNITED-STATES; GENE
Issue Date
201401
Publisher
AMER SOC MICROBIOLOGY
Citation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.80, no.1, pp.366 - 373
Abstract
It is important to rapidly and selectively detect and analyze pathogenic Salmonella enterica subsp. enterica in contaminated food to reduce the morbidity and mortality of Salmonella infection and to guarantee food safety. In the present work, we developed an oligonucleotide microarray containing duplicate specific capture probes based on the carB gene, which encodes the carbamoyl phosphate synthetase large subunit, as a competent biomarker evaluated by genetic analysis to selectively and efficiently detect and discriminate three S. enterica subsp. enterica serotypes: Choleraesuis, Enteritidis, and Typhimurium. Using the developed microarray system, three serotype targets were successfully analyzed in a range as low as 1.6 to 3.1 nM and were specifically discriminated from each other without nonspecific signals. In addition, the constructed microarray did not have cross-reactivity with other common pathogenic bacteria and even enabled the clear discrimination of the target Salmonella serotype from a bacterial mixture. Therefore, these results demonstrated that our novel carB-based oligonucleotide microarray can be used as an effective and specific detection system for S. enterica subsp. enterica serotypes.
URI
http://hdl.handle.net/YU.REPOSITORY/33489http://dx.doi.org/10.1128/AEM.02978-13
ISSN
0099-2240
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공과대학 > 화학공학부 > Articles
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