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dc.contributor.author정태완ko
dc.contributor.author샘매튜ko
dc.contributor.author윤형돈[윤형돈]ko
dc.contributor.author배한섭[배한섭]ko
dc.contributor.authorNadarajan[Nadarajan]ko
dc.date.accessioned2015-12-17T05:04:00Z-
dc.date.available2015-12-17T05:04:00Z-
dc.date.created2015-11-13-
dc.date.issued201502-
dc.identifier.citationJOURNAL OF BIOTECHNOLOGY, v.196, pp.1 - 8-
dc.identifier.issn0168-1656-
dc.identifier.urihttp://hdl.handle.net/YU.REPOSITORY/33444-
dc.identifier.urihttp://dx.doi.org/10.1016/j.jbiotec.2015.01.011-
dc.description.abstractOptically pure beta-amino acids are of high pharmacological significance since they are used as key ingredients in many physiologically active compounds. Despite a number of enzymatic routes to these compounds, an efficient synthesis of beta-amino acids continues to pose a major challenge for researchers. omega-Transaminase has emerged as an important class of enzymes for generating amine compounds. However, only a few omega-transaminases have been reported so far which show activity towards aromatic beta-amino acids. In this study, (S)-omega-transaminase from Burkholderia graminis C4D1M has been functionally characterized and used for the production of chiral aromatic beta-amino acids via kinetic resolution. The enzyme showed a specific activity of 3.1 U/mg towards rac-beta-phenylalanine at 37 degrees C. The K-m and K-cat values of this enzyme towards rac-beta-phenylalanine with pyruvate as the amino acceptor were 2.88 mM and 91.57 min(-1) respectively. Using this enzyme, racemic beta-amino acids were kinetically resolved to produce (R)-beta-amino acids with an excellent enantiomeric excess (> 99%) and similar to 50% conversion. Additionally, kinetic resolution of aromatic beta-amino acids was performed using benzaldehyde as a cheapamino acceptor. (C)2015 Elsevier B.V. All rights reserved.-
dc.language영어-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectORGANIC-SOLVENTS-
dc.subjectAMINOTRANSFERASE-
dc.subjectCLONING-
dc.titleProduction of chiral beta-amino acids using omega-transaminase from Burkholderia graminis-
dc.typeArticle-
dc.identifier.wosid000349964300001-
dc.identifier.scopusid2-s2.0-84921914836-
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