Temperature sensing using red fluorescent protein

Title
Temperature sensing using red fluorescent protein
Author(s)
카나가벨디판쿠마Saravanan Prabhu Nadarajan[Saravanan Prabhu Nadarajan]백광현최권영[최권영]윤형돈[윤형돈]
Keywords
NONCANONICAL AMINO-ACIDS; LIVING CELLS; CONFORMATIONAL-CHANGES; NANOTHERMOMETERS; THERMOGENESIS; THERMOMETRY; DEPENDENCE; SYSTEMS; SAMPLES; DYE
Issue Date
201502
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.20, no.1, pp.67 - 72
Abstract
Genetically encoded fluorescent proteins are extensively utilized for labeling and imaging proteins, organelles, cell tissues, and whole organisms. In this study, we explored the feasibility of mRFP1 and its variants for measuring intracellular temperature. A linear relationship was observed between the temperature and fluorescence intensity of mRFP1 and its variants. Temperature sensitivities of E. coli expressing mRFP1, mRFP-P63A and mRFP-P63A[(4R)-FP] were -1.27%, -1.26% and -0.77%/A degrees C, respectively. Finally, we demonstrated the potentiality of mRFP1 and its variants as an in vivo temperature sensor.
URI
http://hdl.handle.net/YU.REPOSITORY/33425http://dx.doi.org/10.1007/s12257-014-0456-z
ISSN
1226-8372
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생명공학부 > 생명공학부 > Articles
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