Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-alpha factors (LITAFs) from rock bream, Oplegnathus fasciatus

Title
Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-alpha factors (LITAFs) from rock bream, Oplegnathus fasciatus
Author(s)
심상희박찬일[박찬일]황성돈[황성돈]권문경[권문경]최영선[최영선]심원준[심원준]정지현[정지현]김주원[김주원]
Keywords
TUMOR-NECROSIS-FACTOR; NF-KAPPA-B; GRASS CARP REOVIRUS; TRANSCRIPTION FACTOR; INNATE IMMUNITY; SIGNALING PATHWAY; GENE-EXPRESSION; LPS; CACHECTIN; PROTEIN
Issue Date
201402
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation
FISH & SHELLFISH IMMUNOLOGY, v.36, no.2, pp.467 - 474
Abstract
Lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha factor (LITAF) plays an important role controlling the expression of TNF-alpha and the other cytokine genes in the presence of LPS. However, two LITAF homologues have not been characterized in fish. In this study, we cloned two distinct LITAF (RbLITAF1 and RbLITAF2) cDNAs from rock bream (Oplegnathus fasciatus) and characterized their expression profiles after infection with Edwardsiella tarda, Streptococcus iniae or red seabream iridovirus (RSIV). The coding regions of RbLITAF1 and RbLITAF2 cDNAs were 492 bp and 417 bp, encoding 153 and 138 amino acid residues, respectively. The genes consisted of a LITAF domain. RbLITAF1 was highly expressed in the spleen and heart of healthy rock bream, whereas RbLITAF2 was highly expressed in the gill, intestine and stomach. In spleen, the gene expression of RbLITAF1 and RbLITAF2 were increased until 5 days post-infection (dpi), and then decreased at 7 dpi. In kidney, E. tarda and RSIV infection led to induction of the RbLITAF1 gene at 1 dpi, RbLITAF2 gene was down-regulated after pathogen infection. These results suggest that RbLITAFs may be involved in the LITAF-mediated immune response and regulate systemic immune responses against pathogen infection. (C) 2014 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/33274http://dx.doi.org/10.1016/j.fsi.2013.12.023
ISSN
1050-4648
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생명공학부 > 생명공학부 > Articles
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