Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing

Title
Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
Author(s)
딥티싱돌리싱최순모조선미Rakesh Mohan Painuli[Rakesh Mohan Painuli]권성원[권성원]한성수
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY; AYURVEDIC FORMULATION; HYDROLYZABLE TANNINS; IN-VITRO; ANTIOXIDANT; CONSTITUENTS; TRIPHALA; FRUITS; COMBRETACEAE; INHIBITION
Issue Date
201402
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
Abstract
Terminalia chebula is one of the traditional medicines used in the treatment of many diseases. In the present work, different concentrations of various organic and aqueous extracts (solvent-free) of T. chebula were tested on fibroblast (L929) and keratinocytes cells to evaluate its biocompatible concentration by using MTT and live-dead viability/cytotoxic assay. These extracts were found to be effective in decreasing the ammonia accumulation in the media, thereby reducing its toxic effect on cells. DPPH assay further confirmed the free-radical scavenging ability of the extracts which increased with the increase in concentration of each extract. Cell proliferation/apoptosis, cytoskeletal structure, and ECM production were further evaluated by live-dead assay and phalloidin/cytokeratin staining, respectively. The cytoskeletal structure and ECM secretion of the cells treated with extracts showed higher cellular activity in comparison to control. In conclusion, we have demonstrated the effect of these extracts of T. chebula on both types of skin cells and optimized concentration in which it could be used as a bioactive component for wound healing applications by increasing cell proliferation and decreasing free-radical production without affecting the normal cellular matrix. It can also find applications in other therapeutics applications where ammonia toxicity is a limiting factor.
URI
http://hdl.handle.net/YU.REPOSITORY/33237http://dx.doi.org/10.1155/2014/701656
ISSN
1741-427X
Appears in Collections:
중앙도서관 > rims journal
공과대학 > 화학공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE