Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.)

Title
Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.)
Author(s)
배한홍리테쉬고쉬최보성조병관[조병관]임현섭[임현섭]박상언[박상언]배현종[배현종]Savithiry Natarajan, Savithiry [Savithiry Natarajan, Savithiry ]
Keywords
LIGNIN BIOSYNTHETIC-PATHWAY; TRANSCRIPTIONAL ANALYSIS; MONOLIGNOL BIOSYNTHESIS; ARABIDOPSIS-THALIANA; SIGNAL-TRANSDUCTION; CAFFEOYL-COENZYME; DROUGHT STRESSES; ABIOTIC STRESSES; PLANTS; CLONING
Issue Date
201402
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
SCIENTIFIC WORLD JOURNAL
Abstract
Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020- bp open reading frame (ORF), encoding 339 amino acids of 37.37 kDa, with an isoelectric point (pI) of 6.27 (JX524276, HcCCR2). BLAST result found that it has high homology with other plant CCR orthologs. Multiple alignment with other plant CCR sequences showed that it contains two highly conserved motifs: NAD(P) binding domain (VTGAGGFIASWMVKLLLEKGY) at N- terminal and probable catalytic domain (NWYCYGK). According to phylogenetic analysis, it was closely related to CCR sequences of Gossypium hirsutum (ACQ59094) and Populus trichocarpa (CAC07424). HcCCR2 showed ubiquitous expression in various kenaf tissues and the highest expression was detected in mature flower. HcCCR2 was expressed differentially in response to various stresses, and the highest expression was observed by drought and NaCl treatments.
URI
http://hdl.handle.net/YU.REPOSITORY/33224http://dx.doi.org/10.1155/2014/601845
ISSN
1537-744X
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생명공학부 > 생명공학부 > Articles
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