Size effect on transfection and cytotoxicity of nanoscale plasmid DNA/polyethyleneimine complexes for aerosol gene delivery

Title
Size effect on transfection and cytotoxicity of nanoscale plasmid DNA/polyethyleneimine complexes for aerosol gene delivery
Author(s)
변정훈김장우[김장우]
Keywords
GAS-PHASE SYNTHESIS; IN-VITRO; MODIFIED POLYETHYLENIMINES; PDNA/PEI COMPLEXES; JET NEBULIZATION; THERAPY; LUNG; EXPRESSION; DNA; NANOPARTICLES
Issue Date
201402
Publisher
AMER INST PHYSICS
Citation
APPLIED PHYSICS LETTERS, v.104, no.5
Abstract
Nanoscale plasmid DNA (pDNA)/polyethyleneimine (PEI) complexes were fabricated in the aerosol state using a nebulization system consisting of a collison atomizer and a cool-walled diffusion dryer. The aerosol fabricated nanoscale complexes were collected and employed to determine fundamental properties of the complexes, such as size, structure, surface charge, and in vitro gene transfection efficiency and cytotoxicity. The results showed that mass ratio between pDNA and PEI should be optimized to enhance gene transfection efficiency without a significant loss of cell viability. These findings may support practical advancements in the field of nonviral gene delivery. (C) 2014 AIP Publishing LLC.
URI
http://hdl.handle.net/YU.REPOSITORY/33220http://dx.doi.org/10.1063/1.4864268
ISSN
0003-6951
Appears in Collections:
공과대학 > 기계공학부 > Articles
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