Sequence-specific interaction between ABD-B homeodomain and castor gene in Drosophila

Title
Sequence-specific interaction between ABD-B homeodomain and castor gene in Drosophila
Author(s)
유시욱김건희
Keywords
ABDOMINAL-B; BITHORAX-COMPLEX; BINDING-SPECIFICITY; HOX GENES; DNA; PROTEIN; ULTRABITHORAX; RECOGNITION; EXPRESSION; EVOLUTION
Issue Date
201402
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS, v.47, no.2, pp.92 - 97
Abstract
We have examined the effect of bithorax complex genes on the expression of castor gene. During the embryonic stages 12-15, both Ultrabithorax and abdominal-A regulated the castor gene expression negatively, whereas Abdominal-B showed a positive correlation with the castor gene expression according to real-time PCR. To investigate whether ABD-B protein directly interacts with the castor gene, electrophoretic mobility shift assays were performed using the recombinant ABD-B homeodomain and oligonucleotides, which are located within the region 10 kb upstream of the castor gene. The results show that ABD-B protein directly binds to the castor gene specifically. ABD-B binds more strongly to oligonucleotides containing two 5'-TTAT-3' canonical core motifs than the probe containing the 5'-TTAC-3' motif. In addition, the sequences flanking the core motif are also involved in the protein-DNA interaction. The results demonstrate the importance of HD for direct binding to target sequences to regulate the expression level of the target genes.
URI
http://hdl.handle.net/YU.REPOSITORY/33149http://dx.doi.org/10.5483/BMBRep.2014.47.2.117
ISSN
1976-6696
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교육대학원 > 교육대학원 > Articles
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