Betalain and Betaine Composition of Greenhouse- or Field-Produced Beetroot (Beta vulgaris L.) and Inhibition of HepG2 Cell Proliferation

Title
Betalain and Betaine Composition of Greenhouse- or Field-Produced Beetroot (Beta vulgaris L.) and Inhibition of HepG2 Cell Proliferation
Author(s)
Eun Jin Lee[Eun Jin Lee]Dami An[Dami An]Chau Nguyen[Chau Nguyen]B. Patil[B. Patil]Jeongyun Kim[Jeongyun Kim]유길선
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY; IONIZATION MASS-SPECTROMETRY; RED-BEET; GLYCINE BETAINE; HUMAN-NUTRITION; COMMON FOODS; IDENTIFICATION; PLANTS; ANTHOCYANINS; BIOSYNTHESIS
Issue Date
201402
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.6, pp.1324 - 1331
Abstract
The composition of betalain, red or yellow pigments, and betaine (trimethylglycine or glycinebetaine) of nine beetroot (Beta vulgaris L.) cultivars produced in the greenhouse or field was studied. Inhibition of HepG2 cell proliferation by betanin and betaine was also tested. Four predominant betalains, two betacyanins (betanin and isobetanin) and two betaxanthins (vulgaxanthin I and miraxanthin V), were isolated and quantified. Betanin and vulgaxanthin I were the major compounds in red and yellow beetroot extracts, respectively, and they comprised >90% of the betalain content in the tested cultivars. The total betalain content of beetroots produced from the field was between 650 and 800 mu g/g fresh weight, approximately 25% higher than those from the greenhouse. The betaine content of the beetroot grown in the field was between 3.0 and 4.8 mg/g fresh weight, approximately 20% higher than in plants from the greenhouse. There was great variation among the cultivars with respect to their contents of betalains and betaine. In vitro cancer cell cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay on HepG2 cells after exposure to betanin and betaine at concentrations ranging from 0 to 400 mu g/mL and from 0 to 800 mu g/mL for 48 h, respectively. Betanin resulted in a 49% inhibition of HepG2 cell proliferation at 200 mu g/mL and betaine yielded a 25% inhibition at 800 mu g/mL, implying a higher cytotoxicity of betanin compared with betainc. The results indicated that the contents of health-beneficial compounds in beetroots, betalains and betaine, could be increased by modifying the growing conditions and that betanin and betaine extracted from beetroots had some anticancer effects against HepG2 cells.
URI
http://hdl.handle.net/YU.REPOSITORY/33120http://dx.doi.org/10.1021/jf404648u
ISSN
0021-8561
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자연자원대학 > 원예생명과학과 > Articles
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