Transplantation of Heterospheroids of Islet Cells and Mesenchymal Stem Cells for Effective Angiogenesis and Antiapoptosis

Title
Transplantation of Heterospheroids of Islet Cells and Mesenchymal Stem Cells for Effective Angiogenesis and Antiapoptosis
Author(s)
정지헌신정연[신정연]한진[한진]방석호[방석호]정근재[정근재]Muhammad Haque[Muhammad Haque]Taslim Al-Hial[Taslim Al-Hial]노명경[노명경]변영로[변영로]김병수[김병수]
Keywords
MEDIATED INFLAMMATORY REACTION; INSULIN-SECRETION; PANCREATIC-ISLETS; IN-VITRO; LANGERHANS; SURVIVAL; MICE; MASS; XENOTRANSPLANTATION; OVEREXPRESSION
Issue Date
201503
Publisher
MARY ANN LIEBERT, INC
Citation
TISSUE ENGINEERING PART A, v.21, no.5-6, pp.1024 - 1035
Abstract
Although islet transplantation has been suggested as an alternative therapy for type 1 diabetes, there are efficiency concerns that are attributed to poor engraftment of transplanted islets. Hypoxic condition and delayed vasculogenesis induce necrosis and apoptosis of the transplanted islets. To overcome these limitations in islet transplantation, heterospheroids (HSs), which consist of rat islet cells (ICs) and human bone marrow-derived mesenchymal stem cells (hMSCs), were transplanted to the kidney and liver. The HSs cultured under the hypoxic condition system exhibited a significant increase in antiapoptotic gene expression in ICs. hMSCs in the HSs secreted angiogenic and antiapoptotic proteins. With the HS system, ICs and hMSCs were successfully located in the same area of the liver after transplantation of HSs through the portal vein, whereas the transplantation of islets and the dissociated hMSCs did not result in localization of transplanted ICs and hMSCs in the same area. HS transplantation resulted in an increase in angiogenesis at the transplantation area and a decrease in the apoptosis of transplanted ICs after transplantation into the kidney subcapsule compared with transplantation of islet cell clusters (ICCs). Insulin production levels of ICs were higher in the HS transplantation group compared with the ICC transplantation group. The HS system may be a more efficient transplantation method than the conventional methods for the treatment of type 1 diabetes.
URI
http://hdl.handle.net/YU.REPOSITORY/33027http://dx.doi.org/10.1089/ten.tea.2014.0022
ISSN
1937-3341
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약학대학 > 약학부 > Articles
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