Selective Interaction Between Chloroplast beta-ATPase and TGB1(L88) Retards Severe Symptoms Caused by Alternanthera mosaic virus Infection

Title
Selective Interaction Between Chloroplast beta-ATPase and TGB1(L88) Retards Severe Symptoms Caused by Alternanthera mosaic virus Infection
Author(s)
배한홍서은영[서은영]남지륜[남지륜]김현승[김현승]박영환홍석명[홍석명]Dilip Lakshman[Dilip Lakshman]John Hammond[John Hammond]임현섭[임현섭]
Keywords
TO-CELL MOVEMENT; HC-PRO PROTEIN; COAT PROTEIN; PLANT DEFENSE; IN-PLANTA; TOBACCO; HOST; EXPRESSION; SYNTHASE; TOMATO
Issue Date
201403
Publisher
KOREAN SOC PLANT PATHOLOGY
Citation
PLANT PATHOLOGY JOURNAL, v.30, no.1, pp.58 - 67
Abstract
The multifunctional triple gene block protein 1 (TGB1) of the Potexvirus Alternanthera mosaic virus (AltMV) has been reported to have silencing suppressor, cellto-cell movement, and helicase functions. Yeast two hybrid screening using an Arabidopsis thaliana cDNA library with TGB1 as bait, and co-purification with TGB1 inclusion bodies identified several host proteins which interact with AltMV TGB1. Host protein interactions with TGB1 were confirmed by biomolecular fluorescence complementation, which showed positive TGB1 interaction with mitochondrial ATP synthase delta' chain subunit (ATP synthase delta'), light harvesting chlorophyll-protein complex I subunit A4 (LHCA4), chlorophyll a/b binding protein 1 (LHB1B2), chloroplast-localized IscA-like protein (ATCPISCA), and chloroplast beta-ATPase. However, chloroplast beta-ATPase interacts only with TGB1(L88), and not with weak silencing suppressor TGB1(p88). This selective interaction indicates that chloroplast beta-ATPase is not required for AltiVIV movement and replication; however, TRV silencing of chloroplast beta-ATPase in Nicotiana benthamiana induced severe tissue necrosis when plants were infected by AltMV TGB1(L88) but not AltMV TGB1(p88), suggesting that beta-ATPase selectively responded to TGB1(L88) to induce defense responses.
URI
http://hdl.handle.net/YU.REPOSITORY/32856http://dx.doi.org/10.5423/PPJ.OA.09.2013.0097
ISSN
1598-2254
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생명공학부 > 생명공학부 > Articles
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