Bactericidal Effect of Extracts and Metabolites of Robinia pseudoacacia L. on Streptococcus mutans and Porphyromonas gingivalis Causing Dental Plaque and Periodontal Inflammatory Diseases

Title
Bactericidal Effect of Extracts and Metabolites of Robinia pseudoacacia L. on Streptococcus mutans and Porphyromonas gingivalis Causing Dental Plaque and Periodontal Inflammatory Diseases
Author(s)
백광현자얀타쿠마파트라김은실[김은실]오경희[오경희]김현정[김현정]다칼라디카김양선[김양선]
Keywords
VITRO ANTIMICROBIAL ACTIVITIES; GRAM-NEGATIVE BACTERIA; ANTIBACTERIAL ACTIVITY; GROWTH; PLANTS
Issue Date
201504
Publisher
MDPI AG
Citation
MOLECULES, v.20, no.4, pp.6128 - 6139
Abstract
The mouth cavity hosts many types of anaerobic bacteria, including Streptococcusmutans and Porphyromonas gingivalis, which cause periodontal inflammatory diseases and dental caries. The present study was conducted to evaluate the antibacterial potential of extracts of Robinia pseudoacacia and its different fractions, as well as some of its natural compounds against oral pathogens and a nonpathogenic reference bacteria, Escherichia coli. The antibacterial activity of the crude extract and the solvent fractions (hexane, chloroform, ethyl acetate and butanol) of R. pseudoacacia were evaluated against S.mutans, P. gingivalis and E. coliDH5 by standard micro-assay procedure using conventional sterile polystyrene microplates. The results showed that the crude extract was more active against P. gingivalis (100% growth inhibition) than against S. mutans (73% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fractions were active against P. gingivalis, with 91 and 97% growth inhibition, respectively, at 0.2 mg/mL. None of seven natural compounds found in R. pseudoacacia exerted an antibacterial effect on P. gingivalis; however, fisetin and myricetin at 8 mu g/mL inhibited the growth of S. mutans by 81% and 86%, respectively. The crude extract of R. pseudoacacia possesses bioactive compounds that could completely control the growth of P. gingivalis. The antibiotic activities of the hexane and chloroform fractions suggest that the active compounds are hydrophobic in nature. The results indicate the effectiveness of the plant in clinical applications for the treatment of dental plaque and periodontal inflammatory diseases and its potential use as disinfectant for various surgical and orthodontic appliances.
URI
http://hdl.handle.net/YU.REPOSITORY/32791http://dx.doi.org/10.3390/molecules20046128
ISSN
1420-3049
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