The coat protein of Alternanthera mosaic virus is the elicitor of a temperature-sensitive systemic necrosis in Nicotiana benthamiana, and interacts with a host boron transporter protein

Title
The coat protein of Alternanthera mosaic virus is the elicitor of a temperature-sensitive systemic necrosis in Nicotiana benthamiana, and interacts with a host boron transporter protein
Author(s)
배한홍임현섭[임현섭]남지륜[남지륜]서은영[서은영]남문[남문]Anna Maria Vaira[Anna Maria Vaira]장찬용[장찬용]이철호[이철호]김홍기[김홍기]Mark Roh[Mark Roh]John Hammond[John Hammond]
Keywords
DEPENDENT RNA-POLYMERASE; PROGRAMMED CELL-DEATH; N-TERMINAL SEGMENT; SINGLE AMINO-ACID; HYPERSENSITIVE RESPONSE; DISEASE RESISTANCE; MEDIATED RESISTANCE; FLUORESCENT PROTEIN; EXTREME RESISTANCE; RX GENE
Issue Date
201403
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
VIROLOGY, v.452, pp.264 - 278
Abstract
Different isolates of Alternanthera mosaic virus (AltMV; Potexvirus), including four infectious clones derived from AltMV-SP, induce distinct systemic symptoms in Nicotiana benthamiana. Virus accumulation was enhanced at 15 degrees C compared to 25 degrees C; severe clone AltMV 3-7 induced systemic necrosis (SN) and plant death at 15 degrees C. No interaction with potexvirus resistance gene Rx was detected, although SN was ablated by silencing of SGT1, as for other cases of potexvirus-induced necrosis. Substitution of AltMV 3-7 coat protein (CPSP) with that from AltMV-Po (CPPo) eliminated SN at 15 degrees C, and ameliorated symptoms in Alternanthera dentata and soybean. Substitution of only two residues from CPPo [either MN(13,14)ID or LA(76,77)IS] efficiently ablated SN in N. benthamiana. CPSP but not CPPo interacted with Arabidopsis boron transporter protein AtBOR1 by yeast two-hybrid assay; N. benthamiana homolog NbBOR1 interacted more strongly with CPSP than CPPo in bimolecular fluorescence complementation, and may affect recognition of CP as an elicitor of SN. Published by Elsevier Inc.
URI
http://hdl.handle.net/YU.REPOSITORY/32776http://dx.doi.org/10.1016/j.virol.2014.01.021
ISSN
0042-6822
Appears in Collections:
생명공학부 > 생명공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE