Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation

Title
Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation
Author(s)
서정현유희영[유희영]송영훈차형준[차형준]황동수[황동수]
Keywords
MYTILUS-EDULIS-L; ATTACHMENT PLAQUE; ESCHERICHIA-COLI; MATRIX PROTEINS; BLOCK-COPOLYMER; BYSSAL THREADS; COLLAGEN; EXPRESSION; INTEGRINS; RGD
Issue Date
201504
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.20, no.2, pp.211 - 217
Abstract
Endurable coating on biomedical substrates is one of the most important issues in biomedical engineering field. Recently, a robust yet reversible coating has found in the cuticle of mussel byssus and the mimicking of the mussel cuticle coating has been considered as a strategy to build up endurable coating on biomedical materials. To date, the only known protein in the cuticle is mussel foot protein-1 (fp-1). To form endurable and bioactive coating on the biomedical surface, the fusion protein of fp-1 and GRGDSP peptide (fp-1-RGD) was genetically designed and produced in E. coli. The fusion protein of fp-1-RGD was successfully expressed as a form of inclusion body and was simply purified by diluted acetic acid extraction with high purity (similar to 95%). Fp-1-RGD was coated on the tissue culture polystyrene (TCPS) and showed better preosteoblast cell proliferation than that of TCPS. Therefore, the marriage of fp-1 and bioactive peptide can be a good strategy to form bioactive and endurable coating in biomedical field.
URI
http://hdl.handle.net/YU.REPOSITORY/32773http://dx.doi.org/10.1007/s12257-014-0621-4
ISSN
1226-8372
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공과대학 > 화학공학부 > Articles
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