The multifaceted roles of the interspecies signalling molecule indole in Agrobacterium tumefaciens

Title
The multifaceted roles of the interspecies signalling molecule indole in Agrobacterium tumefaciens
Author(s)
이진태이진형백광현조무환김용규
Keywords
PSEUDOMONAS-AERUGINOSA VIRULENCE; QUORUM-SENSING INHIBITORS; BIOFILM FORMATION; ESCHERICHIA-COLI; ANTIBIOTIC-RESISTANCE; STIGMATELLA-AURANTIACA; CROWN GALL; BACTERIA; BIOSYNTHESIS; EXPRESSION
Issue Date
201504
Publisher
WILEY-BLACKWELL
Citation
ENVIRONMENTAL MICROBIOLOGY, v.17, no.4, pp.1234 - 1244
Abstract
Bacteria utilize signal molecules to ensure their survival in environmental niches, and indole is an interspecies and interkingdom signalling molecule, which is widespread in the natural environment. In this study, we sought to identify novel roles of indole in soil-borne bacterium Agrobacterium tumefaciens. Agrobacterium tumefaciens was found not to synthesize indole and to degrade it rapidly. The addition of exogenous indole dose-dependently inhibited A.tumefaciens growth and decreased its motility. Surprisingly, indole markedly increased A.tumefaciens biofilm formation on polystyrene, glass and nylon membrane surfaces and enhanced its antibiotic tolerance. Transcriptional analysis showed that indole markedly up-regulated several biofilm-related (celA, cheA, exoR, phoB, flgE, fliR and motA), stress-related genes (clpB, dnaK, gsp, gyrB, marR and soxR) and efflux genes (emrA, norM, and Atu2551) in A.tumefaciens, which partially explained the increased biofilm formation and antibiotic tolerance. In contrast, the plant auxin indole-3-acetic acid did not affect biofilm formation, antibiotic tolerance or gene expression. Interestingly, indole was found to exhibit several similarities with antibiotics, as it inhibited the growth of non-indole-producing bacteria, whereas these bacteria countered its effects by rapidly degrading indole, and by enhancing biofilm formation and antibiotic tolerance.
URI
http://hdl.handle.net/YU.REPOSITORY/32755http://dx.doi.org/10.1111/1462-2920.12560
ISSN
1462-2912
Appears in Collections:
공과대학 > 화학공학부 > Articles
생명공학부 > 생명공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE