Korean Red Ginseng attenuates ethanol-induced steatosis and oxidative stress via AMPK/Sirt1 activation

Title
Korean Red Ginseng attenuates ethanol-induced steatosis and oxidative stress via AMPK/Sirt1 activation
Author(s)
정태천한재윤[한재윤]이상규[이상규]양지혜[양지혜]김선주[김선주]심주희[심주희]김미광[김미광]구세광[구세광]조일제[조일제]기성환[기성환]
Keywords
ALCOHOLIC LIVER-DISEASE; FATTY LIVER; MOLECULAR-MECHANISMS; HEPATIC STEATOSIS; MURINE MODEL; INJURY; MICE; SIRT1; AMPK; RAT
Issue Date
201504
Publisher
KOREAN SOC GINSENG
Citation
JOURNAL OF GINSENG RESEARCH, v.39, no.2, pp.105 - 115
Abstract
Background: Alcoholic steatosis is the earliest and most common liver disease, and may precede the onset of more severe forms of liver injury. Methods: The effect of Korean Red Ginseng extract (RGE) was tested in two murine models of ethanol (EtOH)-feeding and EtOH-treated hepatocytes. Results: Blood biochemistry analysis demonstrated that RGE treatment improved liver function. Histopathology and measurement of hepatic triglyceride content verified the ability of RGE to inhibit fat accumulation. Consistent with this, RGE administration downregulated hepatic lipogenic gene induction and restored hepatic lipolytic gene repression by EtOH. The role of oxidative stress in the pathogenesis of alcoholic liver diseases is well established. Treatment with RGE attenuated EtOH-induced cytochrome P450 2E1, 4-hydroxynonenal, and nitrotyrosine levels. Alcohol consumption also decreased phosphorylation of adenosine monophosphate-activated protein kinase, which was restored by RGE. Moreover, RGE markedly inhibited fat accumulation in EtOH-treated hepatocytes, which correlated with a decrease in sterol regulatory element-binding protein-1 and a commensurate increase in sirtuin 1 and peroxisome proliferator-activated receptor-a expression. Interestingly, the ginsenosides Rb2 and Rd, but not Rb1, significantly inhibited fat accumulation in hepatocytes. Conclusion: These results demonstrate that RGE and its ginsenoside components inhibit alcoholic steatosis and liver injury by adenosine monophosphate-activated protein kinase/sirtuin 1 activation both in vivo and in vitro, suggesting that RGE may have a potential to treat alcoholic liver disease. Copyright (C) 2014, The Korean Society of Ginseng, Published by Elsevier. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/32723http://dx.doi.org/10.1016/j.jgr.2014.09.001
ISSN
1226-8453
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약학대학 > 약학부 > Articles
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