Development of a porcine renal extracellular matrix scaffold as a platform for kidney regeneration

Title
Development of a porcine renal extracellular matrix scaffold as a platform for kidney regeneration
Author(s)
송필현Seock Hwan Choi[Seock Hwan Choi]So Young Chun[So Young Chun]Seon Yeong Chae[Seon Yeong Chae]Jin Rae Kim[Jin Rae Kim]Se Heang Oh[Se Heang Oh]Sung Kwang Chung[Sung Kwang Chung]Jin Ho Lee[Jin Ho Lee]Gyu-Seog Choi[Gyu-Seog Choi]Tae-Hwan Kim[Tae-Hwan Kim]Tae Gyun Kwon[Tae Gyun Kwon]
Keywords
EPIDERMAL-GROWTH-FACTOR; EPITHELIAL-CELLS; ENDOTHELIAL-CELLS; HEART-VALVES; IN-VITRO; DECELLULARIZATION; PODOCYTES; RECONSTRUCTION; PROLIFERATION; CULTURE
Issue Date
201504
Publisher
WILEY-BLACKWELL
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v.103, no.4, pp.1391 - 1403
Abstract
Acellular scaffolds, possessing an intact three-dimensional extracellular matrix (ECM) architecture and biochemical components, are promising for regeneration of complex organs, such as the kidney. We have successfully developed a porcine renal acellular scaffold and analyzed its physical/biochemical characteristics, biocompatibility, and kidney reconstructive potential. Segmented porcine kidney cortexes were treated with either 1% (v/v) Triton X-100 (Triton) or sodium dodecyl sulfate (SDS). Scanning electron microscopy showed both treatments preserved native tissue architecture, including porosity and composition. Swelling behavior was higher in the Triton-treated compared with the SDS-treated scaffold. Maximum compressive strength was lower in the Triton-treated compared with the SDS-treated scaffold. Attenuated total reflective-infrared spectroscopy showed the presence of amide II (NH) in both scaffolds. Furthermore, richer ECM protein and growth factor contents were observed in the Triton-treated compared with SDS-treated scaffold. Primary human kidney cell adherence, viability, and proliferation were enhanced on the Triton-treated scaffold compared with SDS-treated scaffold. Following murine in vivo implantation, tumorigenecity was absent for both scaffolds after 8 weeks and in the Triton-treated scaffold only, glomeruli-like structure formation and neovascularity were observed. We identified 1% Triton X-100 as a more suitable decellularizing agent for porcine renal ECM scaffolds prior to kidney regeneration. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1391-1403, 2015.
URI
http://hdl.handle.net/YU.REPOSITORY/32697http://dx.doi.org/10.1002/jbm.a.35274
ISSN
1549-3296
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의과대학 > 비뇨기과학교실 > Articles
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