Aerosol-Based Fabrication of Modified Chitosans and Their Application for Gene Transfection

Title
Aerosol-Based Fabrication of Modified Chitosans and Their Application for Gene Transfection
Author(s)
변정훈김희권[김희권]David H. Thompson[David H. Thompson]Jeffrey T. Roberts[Jeffrey T. Roberts]
Keywords
GOLD NANOPARTICLES; IN-VITRO; DELIVERY; DRUG; RELEASE; CARRIER; DERIVATIVES; POLYMER; BIOLOGY; CELLS
Issue Date
201404
Publisher
AMER CHEMICAL SOC
Citation
ACS APPLIED MATERIALS & INTERFACES, v.6, no.7, pp.4597 - 4602
Abstract
Modified chitosan nanoparticles were conveniently obtained by a one-step aerosol method, and their potential for gene transfection was investigated. Droplets containing modified chitosans were formed by collison atomization, dried to form solid particles, and collected and studied for potential use as nanocarriers. Modified chitosans consisted of a chitosan backbone and an additional component [covalently attached cholesterol; or blends with poly(L-lysine) (PLL), polyethyleneimine (PEI), or poly(ethylene glycol) (PEG)]. Agarose gel retardation assays confirmed that modified chitosans could associate with plasmid DNA. Even though the average cell viability of cholesterol-chitosan (Ch-Cs) showed a slightly higher cytotoxicity (similar to 90% viability) than that for unmodified chitosan (Cs, similar to 95%), transfection (>7.5 x 10(5) in relative light units (RLU) mg(-1)) was more effective than it was for Cs (similar to 7.6 x 10(4) RLU mg(-1)). The blending of PEI with Cs (i.e., a Cs/PEI) to produce transfection complexes enhanced the transfection efficiency (similar to 1.3 x 10(6) RLU mg(-1)) more than did the addition of PLL (i.e., a Cs/PLL, similar to 9.3 x 10(5) RLU mg(-1)); however, it also resulted in higher cytotoxicity (similar to 86% viability for Cs/PEI vs similar to 94% for Cs/PLL). The average cell viability (similar to 92%) and transfection efficiency (similar to 1.9 x 10(6) RLU mg(-1)) were complemented further by addition of PEG in Cs/PEI complexes (i.e., a Cs/PEI-PEG). This work concludes that gene transfection of Cs can be significantly enhanced by adding cationic polymers during aerosol fabrication without wet chemical modification processes of Cs.
URI
http://hdl.handle.net/YU.REPOSITORY/32592http://dx.doi.org/10.1021/am501069u
ISSN
1944-8244
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공과대학 > 기계공학부 > Articles
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