Quality evaluation of Carthami Flos by HPLC-UV

Title
Quality evaluation of Carthami Flos by HPLC-UV
Author(s)
손종근이응박동근장해연김태인이승호장현욱우미희[우미희]
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY; HYDROXYSAFFLOR-YELLOW; FINGERPRINT ANALYSIS; TINCTORIUS L.; IN-VITRO; PROTECTS; EXTRACT; FLORETS; VIVO
Issue Date
201505
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.38, no.5, pp.776 - 784
Abstract
A HPLC-DAD method was developed for simultaneous determination of four marker compounds, kaempferol-3-O-rutinoside, safflomin A, safflomin B and bidenoside C, in the extract of the flowers of Carthamus tinctorius Linne. Natural samples were extracted in 50 % aqueous methanol by ultra-sonication for 60 min. Marker compounds were separated on a C-18 column by two-step gradient elution of mobile phase (acetonitrile/water) at a flow rate of 0.75 mL/min and detected at 210 nm. The retention times of safflomin A and safflomin B were shifted according to the pH of the mobile phase. The optimized analytical method was validated to confirm linearity, precision, accuracy and stability of marker compounds. The method was successfully employed to analyze 17 natural samples from different regions, and the data matrix was monitored and visualized by principal component analysis. The assay method could be applied for quality control of the flowers of C. tinctorius Linne.
URI
http://hdl.handle.net/YU.REPOSITORY/32463http://dx.doi.org/10.1007/s12272-014-0402-9
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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