Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays

Title
Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays
Author(s)
임지석Ouriel Caen[Ouriel Caen]Jeremy Vrignon[Jeremy Vrignon]Manfred Konrad[Manfred Konrad]Valerie Taly[Valerie Taly]Jean-Christophe Baret[Jean-Christophe Baret]
Keywords
DROPLET-BASED MICROFLUIDICS; SINGLE-ENZYME; DIGITAL PCR; EMULSIONS; SCALE; CHIP; FLOW; DNA; SURFACTANTS; DEVICES
Issue Date
201505
Publisher
AMER INST PHYSICS
Citation
BIOMICROFLUIDICS, v.9, no.3
Abstract
Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of mono-disperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (> 110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of beta-galactosidase in droplets using nine different concentrations of a fluorogenic substrate. (C) 2015 Author(s).
URI
http://hdl.handle.net/YU.REPOSITORY/32427http://dx.doi.org/10.1063/1.4919415
ISSN
1932-1058
Appears in Collections:
공과대학 > 기계공학부 > Articles
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