Preparation and evaluation of 17-allyamino-17-demethoxygeldanamycin (17-AAG)-loaded poly(lactic acid-co-glycolic acid) nanoparticles

Title
Preparation and evaluation of 17-allyamino-17-demethoxygeldanamycin (17-AAG)-loaded poly(lactic acid-co-glycolic acid) nanoparticles
Author(s)
김종오로샨프라단포우델비자이최임순용철순신범수[신범수]최한곤[최한곤]최주연
Keywords
LOADED PLGA NANOPARTICLES; BEHAVIOR IN-VITRO; PHASE-II TRIAL; PROSTATE-CANCER; DRUG-DELIVERY; SOLID TUMORS; RELEASE; FORMULATION; DESIGN; 17-ALLYLAMINO-17-DEMETHOXYGELDANAMYCIN
Issue Date
201505
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.38, no.5, pp.734 - 741
Abstract
In the present study, we developed the novel 17-allyamino-17-demethoxygeldanamycin (17-AAG)-loaded poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles (NPs) using the combination of sodium lauryl sulfate and poloxamer 407 as the anionic and non-ionic surfactant for stabilization. The PLGA NPs were prepared by emulsification/solvent evaporation method. Both the drug/polymer ratio and phase ratio were 1:10 (w/w). The optimized formulation of 17-AAG-loaded PLGA NPs had a particle size and polydispersity index of 151.6 +/- 2.0 and 0.152 +/- 0.010 nm, respectively, which was further supported by TEM image. The encapsulation efficiency and drug loading capacity were 69.9 and 7.0 %, respectively. In vitro release study showed sustained release. When in vitro release data were fitted to Korsmeyer-Peppas model, the n value was 0.468, which suggested that the drug was released by anomalous or non-Fickian diffusion. In addition, 17-AAG-loaded PLGA NPs in 72 h, displayed approximately 60 % cell viability reduction at 10 mu g/ml 17-AAG concentration, in MCF-7 cell lines, indicating sustained release from NPs. Therefore, our results demonstrated that incorporation of 17-AAG into PLGA NPs could provide a novel effective nanocarrier for the treatment of cancer.
URI
http://hdl.handle.net/YU.REPOSITORY/32424http://dx.doi.org/10.1007/s12272-014-0404-7
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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