Visfatin induces MUC8 and MUC5B expression via p38 MAPK/ROS/NF-kappa B in human airway epithelial cells

Title
Visfatin induces MUC8 and MUC5B expression via p38 MAPK/ROS/NF-kappa B in human airway epithelial cells
Author(s)
송시연정은채배창훈최윤석김용대
Keywords
OXIDATIVE STRESS; MMP-2/9 PRODUCTION; ENDOTHELIAL-CELLS; MAPK; ACTIVATION; PATHWAY; PROTEIN
Issue Date
201405
Publisher
BIOMED CENTRAL LTD
Citation
JOURNAL OF BIOMEDICAL SCIENCE, v.21
Abstract
Background: Among a variety of inflammatory mediators, visfatin is a proinflammatory adipocytokine associated with inflammatory reactions in obesity, metabolic syndrome, chronic inflammatory disease, and autoimmune disease. However, the biological role of visfatin in secretion of major mucins in human airway epithelial cells has not been reported. Therefore, this study was conducted in order to investigate the effect and the brief signaling pathway of visfatin on MUC8 and MUC5B expression in human airway epithelial cells. Results: Visfatin significantly induced MUC8 and MUC5B expression. Visfatin significantly activated phosphorylation of p38 MAPK. Treatment with SB203580 (p38 MAPK inhibitor) and knockdown of p38 MAPK by siRNA significantly blocked visfatin-induced MUC8 and MUC5B expression. Visfatin significantly increased ROS formation. Treatment with SB203580 significantly attenuated visfatin-induced ROS formation. Treatment with NAC (ROS scavenger) and DPI (NADPH oxidase inhibitor) significantly attenuated visfatin-induced MUC8 and MUC5B expression. However, treatment with NAC and DPI did not attenuate visfatin-activated phosphorylation of p38 MAPK. Visfatin significantly activated the phosphorylation of NF-kappa B. Treatment with PDTC (NF-kappa B inhibitor) significantly attenuated visfatin-induced MUC8 and MUC5B expression. Conclusions: These results suggest that visfatin induces MUC8 and MUC5B expression through p38 MAPK/ROS/NF-kappa B signaling pathway in human airway epithelial cells.
URI
http://hdl.handle.net/YU.REPOSITORY/32339http://dx.doi.org/10.1186/1423-0127-21-49
ISSN
1021-7770
Appears in Collections:
의과대학 > 이비인후과학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE