Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages

Title
Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages
Author(s)
도경오김병길[김병길]서영배[서영배]배윤위곽태원[곽태원]강현구[강현구]문익재[문익재]황근배[황근배]박소영
Keywords
NONVIRAL GENE DELIVERY; IN-VITRO; TRANSFECTION EFFICIENCY; ENDOCYTIC PATHWAY; MAMMALIAN-CELLS; PROTEIN CORONA; SERUM; LIPOSOMES; SIRNA; EXPRESSION
Issue Date
201405
Publisher
MDPI AG
Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.15, no.5, pp.7293 - 7312
Abstract
Cationic liposomes are broadly used as non-viral vectors to deliver genetic materials that can be used to treat various diseases including cancer. To circumvent problems associated with cationic liposome-mediated delivery systems such as low transfection efficiency and serum-induced inhibition, cholesterol-based cationic lipids have been synthesized that resist the effects of serum. The introduction of an ether-type linkage and extension of the aminopropyl head group on the cholesterol backbone increased the transfection efficiency and DNA binding affinity compared to a carbamoyl-type linkage and a mono aminopropyl head group, respectively. Under optimal conditions, each liposome formulation showed higher transfection efficiency in AGS and Huh-7 cells than commercially available cationic liposomes, particularly in the presence of serum. The following molecular structures were found to have a positive effect on transfection properties: (i) extended aminopropyl head groups for a strong binding affinity to plasmid DNA; (ii) an ether linkage that favors electrostatic binding to plasmid DNA; and (iii) a cholesterol backbone for serum resistance.
URI
http://hdl.handle.net/YU.REPOSITORY/32336http://dx.doi.org/10.3390/ijms15057293
ISSN
1422-0067
Appears in Collections:
의과대학 > 생리학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
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