Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages
- Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages
- 도경오; 김병길[김병길]; 서영배[서영배]; 배윤위; 곽태원[곽태원]; 강현구[강현구]; 문익재[문익재]; 황근배[황근배]; 박소영
- NONVIRAL GENE DELIVERY; IN-VITRO; TRANSFECTION EFFICIENCY; ENDOCYTIC PATHWAY; MAMMALIAN-CELLS; PROTEIN CORONA; SERUM; LIPOSOMES; SIRNA; EXPRESSION
- Issue Date
- MDPI AG
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.15, no.5, pp.7293 - 7312
- Cationic liposomes are broadly used as non-viral vectors to deliver genetic materials that can be used to treat various diseases including cancer. To circumvent problems associated with cationic liposome-mediated delivery systems such as low transfection efficiency and serum-induced inhibition, cholesterol-based cationic lipids have been synthesized that resist the effects of serum. The introduction of an ether-type linkage and extension of the aminopropyl head group on the cholesterol backbone increased the transfection efficiency and DNA binding affinity compared to a carbamoyl-type linkage and a mono aminopropyl head group, respectively. Under optimal conditions, each liposome formulation showed higher transfection efficiency in AGS and Huh-7 cells than commercially available cationic liposomes, particularly in the presence of serum. The following molecular structures were found to have a positive effect on transfection properties: (i) extended aminopropyl head groups for a strong binding affinity to plasmid DNA; (ii) an ether linkage that favors electrostatic binding to plasmid DNA; and (iii) a cholesterol backbone for serum resistance.
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