Thermoresponsive oligomers reduce Escherichia coli O157:H7 biofouling and virulence

Title
Thermoresponsive oligomers reduce Escherichia coli O157:H7 biofouling and virulence
Author(s)
이진태김성철조무환이진형김용규조현섭김진태
Keywords
O157/H7 BIOFILM FORMATION; BIOMEDICAL APPLICATIONS; CAENORHABDITIS-ELEGANS; CURLI PRODUCTION; STAINLESS-STEEL; O157-H7; RESISTANCE; POLY(N-ISOPROPYLACRYLAMIDE); POLY(N-VINYLCAPROLACTAM); HYDROPHOBICITY
Issue Date
201405
Publisher
TAYLOR & FRANCIS LTD
Citation
BIOFOULING, v.30, no.5, pp.627 - 637
Abstract
Thermoresponsive polymers have potential biomedical applications for drug delivery and tissue engineering. Here, two thermoresponsive oligomers were synthesized, viz. oligo(N-isopropylacrylamide) (ONIPAM) and oligo(N-vinylcaprolactam) (OVCL), and their anti-biofouling abilities investigated against enterohemorrhagic E. coli O157:H7, which produces Shiga-like toxins and forms biofilms. Biofilm formation (biofouling) is closely related to E. coli O157:H7 infection and constitutes a major mechanism of antimicrobial resistance. The synthetic OVCL (MW 679) and three commercial OVCLs (up to MW 54,000) at 30 mu g ml(-1) were found to inhibit biofouling by E. coli O157:H7 at 37 degrees C by more than 80% without adversely affecting bacterial growth. The anti-biofouling activity of ONIPAM was weaker than that of OVCL. However, at 25 degrees C, ONIPAM and OVCL did not affect E. coli O157:H7 biofouling. Transcriptional analysis showed that OVCL temperature-dependently downregulated curli genes in E. coli O157:H7, and this finding was in line with observed reductions in fimbriae production and biofouling. In addition, OVCL downregulated the Shiga-like toxin genes stx1 and stx2 in E. coli O157:H7 and attenuated its in vivo virulence in the nematode Caenorhabditis elegans. These results suggest that OVCL has potential use in antivirulence strategies against persistent E. coli O157:H7 infection.
URI
http://hdl.handle.net/YU.REPOSITORY/32331http://dx.doi.org/10.1080/08927014.2014.907402
ISSN
0892-7014
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공과대학 > 화학공학부 > Articles
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