Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

Title
Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds
Author(s)
서정현김창섭강동균[강동균]차형준[차형준]
Keywords
ICE-NUCLEATION PROTEIN; RECOMBINANT ESCHERICHIA-COLI; PSEUDOMONAS-PUTIDA JS444; AMPEROMETRIC MICROBIAL BIOSENSOR; METHYL PARATHION HYDROLASE; CELLULOSE-BINDING DOMAIN; N-TERMINAL DOMAIN; NERVE AGENTS; SURFACE-DISPLAY; SIMULTANEOUS DEGRADATION
Issue Date
201405
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
BIOTECHNOLOGY ADVANCES, v.32, no.3, pp.652 - 662
Abstract
The development of efficient tools is required for the eco-friendly detoxification and effective detection of neurotoxic organophosphates (OPs). Although enzymes have received significant attention as biocatalysts because of their high specific activity, the uneconomic and labor-intensive processes of enzyme production and purification make their broad use in practical applications difficult. Because whole7cell systems offer several advantages compared with free enzymes, including high stability, a reduced purification requirement, and low preparation cost, they have been suggested as promising biocatalysts for the detoxification and detection of OPs. To develop efficient whole-cell biocatalysts with enhanced activity and a broad spectrum of substrate specificity, several factors have been considered, namely the selected strains, the chosen OP-hydrolyzing enzymes, where enzymes are localized in a cell, and which enhancer will assist the expression, function, and folding of the enzyme. In this article, we review the current investigative progress in the development of engineered whole-cell biocatalysts with excellent OP-hydrolyzing activity, a broad spectrum of substrate specificity, and outstanding stability for the detoxification and detection of OPs. (C) 2014 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/32325http://dx.doi.org/10.1016/j.biotechadv.2014.04.010
ISSN
0734-9750
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공과대학 > 화학공학부 > Articles
이과대학 > 화학생화학부 > Articles
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