Effect of Various Intercalators on the Fenton- Type Oxidative Cleavage of Double- Stranded DNA
- Effect of Various Intercalators on the Fenton- Type Oxidative Cleavage of Double- Stranded DNA
- 김석규; 김현정; 성기웅; 김경원; 박종진; Biao, Jin[Biao, Jin]
- CROSS-CORRELATION SPECTROSCOPY; LINEAR DICHROISM; NUCLEIC-ACIDS; BINDING; DAMAGE; FLUORESCENCE; KINETICS; METHIDIUMPROPYL-EDTA.FE(II); ANTIOXIDANTS; COMPLEXES
- Issue Date
- WILEY-V C H VERLAG GMBH
- CHEMISTRY-AN ASIAN JOURNAL, v.9, no.5, pp.1341 - 1348
- The intensity of the linear dichroism (LD) in the absorption region of DNA (about 260nm) decreased with time in the presence of [Fe(EDTA)](2+) (EDTA=ethylenediaminetetraacetic acid), H2O2, and ascorbate. The decrease in the LD signal indicated either an increase in flexibility, a shortening of the DNA stem, or both, owing to oxidative cleavage, and was best described by the difference between the two single-exponential-decay curves, thereby suggesting the involvement of two sequential first-order reactions. The fast reaction was assigned to cleavage of one of two DNA strands, which increased the flexibility of the DNA. The slow reaction corresponded to cleavage at or near the first cleavage site, thereby shortening the DNA stem. The presence of an intercalator, including ethidium, propidium, 9-aminoacridine, and proflavine, inhibited the first step of the cleavage reaction. One of the possible reasons for the observed inhibition might be a change in the DNA conformation near the intercalation site. Intercalation caused an unwinding and elongation of the DNA and resulted in changes in the location of the Hatoms of the sugar moiety, which is known to be the main site at which hydroxyl radicals react.
- Appears in Collections:
- 이과대학 > 화학생화학부 > Articles
문과대학 > 중국언어문화학과 > Articles
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)