Effect of Various Intercalators on the Fenton- Type Oxidative Cleavage of Double- Stranded DNA

Title
Effect of Various Intercalators on the Fenton- Type Oxidative Cleavage of Double- Stranded DNA
Author(s)
김석규김현정성기웅김경원박종진Biao, Jin[Biao, Jin]
Keywords
CROSS-CORRELATION SPECTROSCOPY; LINEAR DICHROISM; NUCLEIC-ACIDS; BINDING; DAMAGE; FLUORESCENCE; KINETICS; METHIDIUMPROPYL-EDTA.FE(II); ANTIOXIDANTS; COMPLEXES
Issue Date
201405
Publisher
WILEY-V C H VERLAG GMBH
Citation
CHEMISTRY-AN ASIAN JOURNAL, v.9, no.5, pp.1341 - 1348
Abstract
The intensity of the linear dichroism (LD) in the absorption region of DNA (about 260nm) decreased with time in the presence of [Fe(EDTA)](2+) (EDTA=ethylenediaminetetraacetic acid), H2O2, and ascorbate. The decrease in the LD signal indicated either an increase in flexibility, a shortening of the DNA stem, or both, owing to oxidative cleavage, and was best described by the difference between the two single-exponential-decay curves, thereby suggesting the involvement of two sequential first-order reactions. The fast reaction was assigned to cleavage of one of two DNA strands, which increased the flexibility of the DNA. The slow reaction corresponded to cleavage at or near the first cleavage site, thereby shortening the DNA stem. The presence of an intercalator, including ethidium, propidium, 9-aminoacridine, and proflavine, inhibited the first step of the cleavage reaction. One of the possible reasons for the observed inhibition might be a change in the DNA conformation near the intercalation site. Intercalation caused an unwinding and elongation of the DNA and resulted in changes in the location of the Hatoms of the sugar moiety, which is known to be the main site at which hydroxyl radicals react.
URI
http://hdl.handle.net/YU.REPOSITORY/32310http://dx.doi.org/10.1002/asia.201400099
ISSN
1861-4728
Appears in Collections:
이과대학 > 화학생화학부 > Articles
문과대학 > 중국언어문화학과 > Articles
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