Mathematical and numerical model to study two-dimensional free flow isoelectric focusing

Title
Mathematical and numerical model to study two-dimensional free flow isoelectric focusing
Author(s)
심재술유기수[유기수]Jin Liu[Jin Liu]Prashanta Dutta[Prashanta Dutta]
Keywords
CAPILLARY-ELECTROPHORESIS; MICROFLUIDIC CHIP; SEPARATION; SIMULATION
Issue Date
201405
Publisher
AMER INST PHYSICS
Citation
BIOMICROFLUIDICS, v.8, no.3
Abstract
Even though isoelectric focusing (IEF) is a very useful technique for sample concentration and separation, it is challenging to extract separated samples for further processing. Moreover, the continuous sample concentration and separation are not possible in the conventional IEF. To overcome these challenges, free flow IEF (FFIEF) is introduced in which a flow field is applied in the direction perpendicular to the applied electric field. In this study, a mathematical model is developed for FFIEF to understand the roles of flow and electric fields for efficient design of microfluidic chip for continuous separation of proteins from an initial well mixed solution. A finite volume based numerical scheme is implemented to simulate two dimensional FFIEF in a microfluidic chip. Simulation results indicate that a pH gradient forms as samples flow downstream and this pH profile agrees well with experimental results validating our model. In addition, our simulation results predict the experimental behavior of pI markers in a FFIEF microchip. This numerical model is used to predict the separation behavior of two proteins (serum albumin and cardiac troponin I) in a two-dimensional straight microchip. The effect of electric field is investigated for continuous separation of proteins. Moreover, a new channel design is presented to increase the separation resolution by introducing cross-stream flow velocity. Numerical results indicate that the separation resolution can be improved by three folds in this new design compare to the conventional straight channel design. (C) 2014 AIP Publishing LLC.
URI
http://hdl.handle.net/YU.REPOSITORY/32241http://dx.doi.org/10.1063/1.4883575
ISSN
1932-1058
Appears in Collections:
공과대학 > 기계공학부 > Articles
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