Improved production of poly(gamma-glutamic acid) by Bacillus subtilis RKY3 and its recovery from viscous fermentation broth as a biodegradable polymer

Title
Improved production of poly(gamma-glutamic acid) by Bacillus subtilis RKY3 and its recovery from viscous fermentation broth as a biodegradable polymer
Author(s)
위영중정귀택[정귀택]김진남[김진남]류화원[류화원]
Keywords
GAMMA-POLYGLUTAMIC ACID; GLUTAMIC-ACID; POLY(GLUTAMIC ACID); LICHENIFORMIS; BIOSYNTHESIS; BIOPOLYMER; POLYPEPTIDE; PH
Issue Date
201405
Publisher
WILEY-BLACKWELL
Citation
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v.89, no.5, pp.728 - 734
Abstract
BACKGROUND Physiological parameters (culture pH, aeration rate, and agitation speed) were optimized to improve gamma-PGA production by batch culture of Bacillus subtilis RKY3. The optimum conditions for recovery of gamma-PGA from highly viscous culture broth were also investigated. RESULTS Enhanced gamma-PGA production (41.4 g L-1, 397 cP) was observed under optimized physiological culture parameters (no pH control, 1.5 L min(-1) aeration, and 300 rpm agitation). The cells were completely removed by diluting the viscous culture broth 7.5-, 5.0-, and 2.5-fold at pH 6.0, 4.0, and 2.0, respectively. Maximum recovery of gamma-PGA was observed when the volume ratio of cell-free culture broth to cold ethanol was 1:5 at pH 6.0. gamma-PGA with a molecular weight kDa was successfully excluded in an ultrafiltration system to develop more economical recovery of gamma-PGA from cell-free culture broth, which resulted in efficient gamma-PGA concentration with a molecular weight >100 kDa. CONCLUSION The physiological parameters affecting gamma-PGA production were optimized, and the isolation and recovery of gamma-PGA from viscous culture broth was also evaluated. Cells were removed completely by diluting the culture broth, and precipitation of gamma-PGA using cold ethanol was better than that of other alcohols. Ultrafiltration was found to be an efficient recovery method for concentration of culture broth containing gamma-PGA. (c) 2013 Society of Chemical Industry
URI
http://hdl.handle.net/YU.REPOSITORY/32208http://dx.doi.org/10.1002/jctb.4178
ISSN
0268-2575
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자연자원대학 > 식품공학과 > Articles
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