Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation

Title
Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation
Author(s)
배한홍권영상[권영상]김상곤[김상곤]정우식[정우식]정성우[정성우]신성철[신성철]정미정[정미정]박수철[박수철]곽연식[곽연식]배동원[배동원]이용복[이용복]
Keywords
RICE SHEATH BLIGHT; PROTEINS
Issue Date
201405
Publisher
ELSEVIER SCI LTD
Citation
FUNGAL BIOLOGY, v.118, no.5-6, pp.433 - 443
Abstract
Rhizoctonia solani (R. solani), a soil-borne necrotrophic pathogen, causes various plant diseases. Rhizoctonia solani is a mitosporic fungus, the sclerotium of which is the primary inoculum and ensures survival of the fungus during the offseason of the host crop. Since the fungus does not produce any asexual or sexual spores, understanding the biology of sclerotia is important to examine pathogen ecology and develop more efficient methods for crop protection. Here, one- and two-dimensional gel electrophoresis (1-DE and 2-DE, respectively) were used to examine protein regulation during the maturation of fungal sclerotia. A total of 75 proteins (20 proteins from 1-DE using matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) and 55 proteins from 2-DE using MALDI-TOF MS or MALDI-TOF/TOF MS) were differentially expressed during sclerotial maturation. The identified proteins were classified into ten categories based on their biological functions, including genetic information processing, carbohydrate metabolism, cell defense, amino acid metabolism, nucleotide metabolism, cellular processes, pathogenicity and mycotoxin production, and hypothetical or unknown functions. Interestingly, two vacuole function-related proteins were highly up-regulated throughout sclerotial maturation, which was confirmed at the transcript level by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. These findings contribute to our understanding of the biology of R. solani sclerotia. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/32189http://dx.doi.org/10.1016/j.funbio.2014.02.001
ISSN
1878-6146
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생명공학부 > 생명공학부 > Articles
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