Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine

Title
Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine
Author(s)
김화영김문정이병천[이병천]황광연[황광연]Gladyshev VN[Gladyshev VN]
Keywords
ESCHERICHIA-COLI; SULFOXIDE REDUCTASES; GLYCINE REDUCTASE; ACTIVE-SITE; INSERTION; MECHANISM; CYSTEINE; SECIS
Issue Date
201506
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.461, no.4, pp.648 - 652
Abstract
Thioredoxin (Trx) is a major thiol-disulfide reductase that plays a role in many biological processes, including DNA replication and redox signaling. Although selenocysteine (Sec)-containing Trxs have been identified in certain bacteria, their enzymatic properties have not been characterized. In this study, we expressed a selenoprotein Trx from Treponema denticola, an oral spirochete, in Escherichia colt and characterized this selenoenzyme and its natural cysteine (Cys) homologue using E. coli Trx1 as a positive control. Se-75 metabolic labeling and mutation analyses showed that the SECIS (Sec insertion sequence) of T. denticola selenoprotein Trx is functional in the E. coli Sec insertion system with specific selenium incorporation into the Sec residue. The selenoprotein Trx exhibited approximately 10-fold higher catalytic activity than the Sec-to-Cys version and natural Cys homologue and E. coli Trx1, suggesting that Sec confers higher catalytic activity on this thiol-disulfide reductase. Kinetic analysis also showed that the selenoprotein Trx had a 30-fold higher K-m than Cys-containing homologues, suggesting that this selenoenzyme is adapted to work efficiently with high concentrations of substrate. Collectively, the results of this study support the hypothesis that selenium utilization in oxidoreductase systems is primarily due to the catalytic advantage provided by the rare amino acid, Sec. (C) 2015 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/32137http://dx.doi.org/10.1016/j.bbrc.2015.04.082
ISSN
0006-291X
Appears in Collections:
의과대학 > 생화학.분자생물학교실 > Articles
의과대학 > 영상의학과학교실 > Articles
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