Polypeptide-based Micelles for Delivery of Irinotecan: Physicochemical and In vivo Characterization

Title
Polypeptide-based Micelles for Delivery of Irinotecan: Physicochemical and In vivo Characterization
Author(s)
김종오티르거네쉬라마사미최주연조혁준우마데비[우마데비]신범수[신범수]최한곤[최한곤]용철순
Keywords
BLOCK IONOMER COMPLEXES; DRUG-DELIVERY; BREAST-CANCER; DOXORUBICIN; NANOPARTICLES; CAMPTOTHECIN; EFFICACY; FORMULATION; COPOLYMERS; LIPOSOMES
Issue Date
201506
Publisher
SPRINGER/PLENUM PUBLISHERS
Citation
PHARMACEUTICAL RESEARCH, v.32, no.6, pp.1947 - 1956
Abstract
Irinotecan (IRI) is a broad spectrum chemotherapeutic agent used individually or in combination to treat multiple malignancies. Present study aimed at developing polypeptide-based block ionomer complex (BIC) micelles to improve the pharmacokinetic and antitumor response of IRI. Irinotecan-loaded BIC micelles (IRI-BIC) was prepared and evaluated in terms of various physicochemical and biological parameters including size, shape, release, cytotoxicity, and pharmacokinetic analysis. In vivo antitumor efficacy was investigated in SCC-7 bearing xenograft tumor model. IRI was successfully incorporated into the ionic cores of poly(ethylene glycol)-b-poly(aspartic acid) (PEG-b-PAA) with a high drug loading capacity (80%). The electrostatically assembled BIC micelles were nanosized (50 nm) with uniform size distribution pattern (PDI0.1). The BIC micelles exhibited pH-sensitiveness with limited release of IRI at physiological conditions and significantly enhanced the release rate at acidic conditions, making it an ideal delivery system for tumor targeting. The IRI-BIC showed a dose-dependent cytotoxicity in SCC-7 and A-549 cancer cell lines. Pharmacokinetic studies clearly showed that BIC micelles improved the IRI blood circulation time and decreased its elimination rate constant, while that of free IRI, rapidly eliminated from the central compartment. Moreover, IRI-BIC showed superior therapeutic performance with no toxicity in BALB/c nude xenograft mice. The micelle treated group showed an inhibition rate of 66% compared to free IRI treated group. Taken together, BIC micelles could be a potentially useful nanovehicle with promising applicability in systemic tumor treatment.
URI
http://hdl.handle.net/YU.REPOSITORY/32053http://dx.doi.org/10.1007/s11095-014-1588-8
ISSN
0724-8741
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약학대학 > 약학부 > Articles
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