IN VIVO IMMUNOMODULATORY EFFECTS OF THE METHANOLIC LEAF EXTRACT OF GYMNEMA SYLVESTRE IN SWISS ALBINO MICE

Title
IN VIVO IMMUNOMODULATORY EFFECTS OF THE METHANOLIC LEAF EXTRACT OF GYMNEMA SYLVESTRE IN SWISS ALBINO MICE
Author(s)
슈리티슈클라Laxmi Ahirwal [Laxmi Ahirwal ]Siddhartha Singh [Siddhartha Singh ]Vandana Bharti[Vandana Bharti]Archana Mehta[Archana Mehta]Manish Kumar Dubey[Manish Kumar Dubey]
Keywords
T-LYMPHOCYTES; AGENTS
Issue Date
201506
Publisher
INST BIOLOSKA ISTRAZIVANJA SINISA STANKOVIC
Citation
ARCHIVES OF BIOLOGICAL SCIENCES, v.67, no.2, pp.561 - 570
Abstract
In the present study we performed a comparative phytochemical analysis of the immunomodulating activities of the methanol leaf extract of Gymnema sylvestre (MLEGS) in Swiss albino mice. The phytochemical screening conducted on MLEGS revealed the presence of several phytoconstituents, including saponins, alkaloids, glycosides, phenols, tannins, and flavonoids. Immunomodulatory activities were determined by hemagglutination antibody (HA) titer and delayed-type hypersensitivity (DTH) tests for determining specific and non-specific immune responses. Flow cytometric techniques were performed for the estimation of B lymphocytes (CD3 and CD19) and Th2 cytokines (IL-2, IFN-gamma and IL-4). The response produced by oral administration of MLEGS elicited a significant reduction in a dose-related manner in the primary and secondary antibody response and DTH response. The response produced by oral administration of MLEGS caused a significant reduction in a dose-related manner in the primary and secondary antibody and DTH responses, with maximum reduction observed at 200 mg/kg-body wt. The maximal reductions in the production of CD3, CD19, IL-2, IFN-gamma and IL-4 were 31.59, 32.12, 29.51, 32.45 and 33.53%, respectively, at 200 mg/kg body weight. This study demonstrates that G. sylvestre exerts immunosuppressive effects on the components of the immune system of mice, and points to its significant immunomodulatory potential.
URI
http://hdl.handle.net/YU.REPOSITORY/32037http://dx.doi.org/10.2298/ABS141027018A
ISSN
0354-4664
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중앙도서관 > rims journal
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