Stabilization of a hyaluronate-associated gene delivery system using calcium ions

Title
Stabilization of a hyaluronate-associated gene delivery system using calcium ions
Author(s)
도경오Min Feng[Min Feng]Basma M. Ibrahim[Basma M. Ibrahim]Erin M. Wilson[Erin M. Wilson]Brandon K. Bergman[Brandon K. Bergman]Christopher Park[Christopher Park]Yoon Yeo[Yoon Yeo]
Keywords
PLASMID-LIPID PARTICLES; EGG-BOX MODEL; DIVALENT-CATIONS; MAMMALIAN-CELLS; SIRNA DELIVERY; TRANSFECTION; ACID; CONFORMATION; COMPLEXES; GELATION
Issue Date
201406
Publisher
ROYAL SOC CHEMISTRY
Citation
BIOMATERIALS SCIENCE, v.2, no.6, pp.936 - 942
Abstract
A "DPH" ternary complex consisting of plasmid DNA (pDNA), intracellularly degradable polyethyleneimine, and hyaluronic acid (HA) is a promising non-viral gene carrier with low toxicity and good gene transfection efficiency. HA plays a key role in providing an optimal balance between DNA protection and release, but it causes aggregation due to the entanglement of HA chains of neighbouring DPH particles. Here we report that the addition of an optimal level of Ca2+ successfully prevents particle aggregation and maintains a relatively small size. The Ca-stabilized DPH is comparable to DPH in cytotoxicity and gene transfection efficiency. MW monitoring and conductometric titration suggest that such size stabilization effect is partly mediated by the complexation between HA and Ca2+, which enables intra-and intermolecular interactions of HAs.
URI
http://hdl.handle.net/YU.REPOSITORY/32029http://dx.doi.org/10.1039/c4bm00012a
ISSN
2047-4830
Appears in Collections:
의과대학 > 생리학교실 > Articles
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