Interaction of Metallo- and free base meso-tetrakis(N-methylpyridium-4-yl)porphyrin with a G-quadruplex: Effect of the central metal ions

Title
Interaction of Metallo- and free base meso-tetrakis(N-methylpyridium-4-yl)porphyrin with a G-quadruplex: Effect of the central metal ions
Author(s)
김석규김윤화이창윤정세채[정세채]
Keywords
CATIONIC PORPHYRINS; NUCLEIC-ACIDS; GUANINE QUADRUPLEXES; ENERGY-TRANSFER; SQUARE-PLANAR; END-STACKING; BINDING MODE; SIDE ARMS; DNA; COMPLEXES
Issue Date
201406
Publisher
ELSEVIER SCIENCE BV
Citation
BIOPHYSICAL CHEMISTRY, v.190, pp.17 - 24
Abstract
The effects of the central metal ion on complex formation between meso-tetrakis(N-methylpyridium-4-yl)porphyrin (TIVIPyP) and the thrombin-binding aptamer G-quadruplex, 5'G(2)T(2)G(2)TGTG(2)T(2)G(2), were examined in this study. The central metal ions were vanadium and zinc. At a [porphyrin]/[G-quadruplex] ratio of less than one, the absorption and CD spectra were unaffected by the mixing ratio for all three porphyrins, suggesting that the binding mode is homogeneous. Relatively small changes in the absorption spectrum when forming the complexes with the G-quadruplex, the positive CD signal, and the large accessibility of the I- quencher, suggested that all these porphyrins are not intercalated between the G-quartet. Stabilization of the G-quadruplex by ZnTMPyP was most effective. The effect of VOTMPyP on G-quadruplex stabilization was moderate, whereas TMPyP slightly destabilized G-quadruplex. From this observation, the involvement of the ligation of one G-quartet component to the central metal ion in G-quadruplex stabilization by metallo-TMPyP is suggested (C) 2014 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/32012http://dx.doi.org/10.1016/j.bpc.2014.03.005
ISSN
0301-4622
Appears in Collections:
이과대학 > 화학생화학부 > Articles
문과대학 > 중국언어문화학과 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE