Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA

Title
Biotransformation of Indole to 3-Methylindole by Lysinibacillus xylanilyticus Strain MA
Author(s)
어로라펀커즈쿠말Kartik Dhar[Kartik Dhar]Rafael Alejandro Veloz Garc?a[Rafael Alejandro Veloz Garc?a]Ashutosh Sharma[Ashutosh Sharma]
Keywords
SKATOLE; PATHWAY; DEGRADATION; BACTERIA
Issue Date
201506
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
JOURNAL OF CHEMISTRY
Abstract
An indole-biotransforming strain MA was identified as Lysinibacillus xylanilyticus on the basis of the 16S rRNA gene sequencing. It transforms indole completely from the broth culture in the presence of an additional carbon source ( i.e., sodium succinate). Gas-chromatography-mass spectrometry identified indole-3-acetamide, indole-3-acetic acid, and 3-methylindole as transformation products. Tryptophan-2-monooxygenase activity was detected in the crude extracts of indole-induced cells of strain MA, which confirms the formation of indole-3-acetamide from tryptophan in the degradation pathway of indole. On the basis of identified metabolites and enzyme assay, we have proposed a new transformation pathway for indole degradation. Indole was first transformed to indole-3-acetamide via tryptophan. Indole-3-acetamide was then transformed to indole-3-acetic acid that was decarboxylated to 3-methylindole. This is the first report of a 3-methylindole synthesis via the degradation pathway of indole.
URI
http://hdl.handle.net/YU.REPOSITORY/31998http://dx.doi.org/10.1155/2015/425329
ISSN
2090-9063
Appears in Collections:
중앙도서관 > rims journal
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