Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene

Title
Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene
Author(s)
백광현모히둘하산김현순[김현순]전재흥[전재흥]김성홍[김성홍]문보경[문보경]심상희송재영[송재영]
Keywords
TAXOL BIOSYNTHETIC GENES; HAIRY ROOT CULTURES; ESCHERICHIA-COLI; COMMITTED STEP; PACIFIC YEW; TAXUS-MEDIA; SUSPENSION-CULTURES; DITERPENE CYCLASE; SYNTHASE GENE; BACCATIN-III
Issue Date
201406
Publisher
SPRINGER
Citation
PLANT CELL REPORTS, v.33, no.6, pp.895 - 904
Abstract
We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(A (R))) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been used extensively to treat various types of cancer. Taxadiene, the first committed product of paclitaxel synthesis is cyclized from geranylgeranyl diphosphate (GGPP), and further complex hydroxylation and acylation processes of the unique taxane core skeleton produce paclitaxel. To accomplish de novo production of taxadiene, we transformed Nicotiana benthamiana with a taxadiene synthase (TS) gene. The introduced TS gene under the transcriptional control of the CaMV 35S promoter was constitutively expressed in N. benthamiana, and the de novo production of taxadiene was confirmed by mass spectroscopy profiling. Transformed N. benthamiana homozygous lines produced 11-27 mu g taxadiene/g of dry weight. The highest taxadiene production line TSS-8 was further treated with an elicitor, methyl jasmonate, and metabolic pathway shunting by suppression of the phytoene synthase gene expression which resulted in accumulation of increased taxadiene accumulation by 1.4- or 1.9-fold, respectively. In summary, we report that the production of taxadiene in N. benthamiana was possible by the ectopic expression of the TS gene, and higher accumulation of taxadiene could be achieved by elicitor treatment or metabolic pathway shunting of the terpenoid pathway.
URI
http://hdl.handle.net/YU.REPOSITORY/31970http://dx.doi.org/10.1007/s00299-014-1568-9
ISSN
0721-7714
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생명공학부 > 생명공학부 > Articles
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