ISOLATION AND IDENTIFICATION OF Aspergillus flavus FROM POULTRY FEED SAMPLES USING COMBINED TRADITIONAL-MOLECULAR APPROACH AND EXPRESSION OF CYP64A1 AT mRNA LEVEL

Title
ISOLATION AND IDENTIFICATION OF Aspergillus flavus FROM POULTRY FEED SAMPLES USING COMBINED TRADITIONAL-MOLECULAR APPROACH AND EXPRESSION OF CYP64A1 AT mRNA LEVEL
Author(s)
나즈물후세인프라딥라즈Jayedul Hassan[Jayedul Hassan]Hyungdon Yun[Hyungdon Yun]
Keywords
TRANSCRIBED SPACER REGIONS; POLYMERASE-CHAIN-REACTION; PCR-BASED DETECTION; PHANEROCHAETE-CHRYSOSPORIUM; AFLATOXIN B-1; MYCOTOXINS; PARASITICUS; FUNGI; MYCOBIOTA; DIVERSITY
Issue Date
201406
Publisher
UNIV AGRICULTURE, FAC VETERINARY SCIENCE
Citation
PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES, v.51, no.2, pp.287 - 291
Abstract
The research work was aimed for isolation and identification of Aspergillus flavus from poultry feed samples using conventional and molecular techniques. Out of 23 samples, 8 (34.78%) were found positive for A. flavus. The molecular techniques comprise comparison of ITS1-5.8S-ITS2 region. In addition, the ability of the isolates to express aflatoxin synthase (CYP64A1) gene in synthetic liquid medium was also analyzed. Based on cultural characteristics, unique nature of the ITS 1 and 2 genes and expression of CYP64A1 at mRNA level, A. flavus was accurately identified from the feed samples. The isolated A. flavus would be a potential candidate for further studies and application.
URI
http://hdl.handle.net/YU.REPOSITORY/31922
ISSN
0552-9034
Appears in Collections:
중앙도서관 > rims journal
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