Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells

Title
Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells
Author(s)
장현욱정윤정[정윤정]최용수[최용수]신재문[신재문]조현지[조현지]강정한[강정한]박관규[박관규]채정윤[채정윤]배영석[배영석]한상미[한상미]김철호[김철호]장영채[장영채]
Keywords
FOCAL-ADHESION KINASE; NF-KAPPA-B; BEE VENOM; MMP-9 EXPRESSION; CARCINOMA-CELLS; OVARIAN-CANCER; MIGRATION; ACTIVATION; INDUCTION; RECEPTOR
Issue Date
201406
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.68, pp.218 - 225
Abstract
Bee venom is a natural compound produced by the honey bee (Apis mellifera), and has been reported as having the biological and pharmacological activities, including anti-bacterial, anti-viral and anti-inflammation. In the present study, the inhibitory effects of bee venom and its major peptide components on the tumor invasion were demonstrated. It was confirmed the inhibitory effects of bee venom, melittin, and apamin on the EGF-induced invasion of breast cancer cells. Transwell invasion and wound-healing assays showed that bee venom and melittin significantly inhibits the EGF-induced invasion and migration of breast cancer cells. Also, bee venom and melittin reduced the EGF-stimulated F-actin reorganization at the leading edge, but apamin did not affect. Particularly, melittin inhibited the EGF-induced MMP-9 expression via blocking the NF-kappa B and PI3K/Akt/mTOR pathway. In addition, melittin significantly suppressed the EGF-induced FAR phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway. These results suggest that inhibitory effects of melittin on breast cancer cell motility and migration may be related to the inhibition of mTOR pathway. (C) 2014 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31879http://dx.doi.org/10.1016/j.fct.2014.03.022
ISSN
0278-6915
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약학대학 > 약학부 > Articles
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