Quality Evaluation and Pattern Recognition Analyses of Bioactive Marker Compounds from Farfarae Flos Using HPLC/PDA

Title
Quality Evaluation and Pattern Recognition Analyses of Bioactive Marker Compounds from Farfarae Flos Using HPLC/PDA
Author(s)
손종근U Min Seo[U Min Seo]Bing Tian Zhao[Bing Tian Zhao]김원일[김원일]서은경[서은경]김재현[김재현]민병선[민병선]신범수[신범수]우미희[우미희]
Keywords
FLOWER BUDS; SESQUITERPENOIDS; CELLS
Issue Date
201507
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
CHEMICAL & PHARMACEUTICAL BULLETIN, v.63, no.7, pp.546 - 553
Abstract
The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mmx4.6-mm, 5-mu m) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos.
URI
http://hdl.handle.net/YU.REPOSITORY/31621
ISSN
0009-2363
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약학대학 > 약학부 > Articles
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