Coumarins reduce biofilm formation and the virulence of Escherichia coli O157:H7

Title
Coumarins reduce biofilm formation and the virulence of Escherichia coli O157:H7
Author(s)
이진태조무환이진형김용규조현섭유시용[유시용]
Keywords
CAENORHABDITIS-ELEGANS; PSEUDOMONAS-AERUGINOSA; INHIBITION; O157-H7; MOTILITY; FLAGELLA; O157/H7; INFECTIONS; SYSTEM
Issue Date
201407
Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Citation
PHYTOMEDICINE, v.21, no.8-9, pp.1037 - 1042
Abstract
E. coli O157:H7 is the most common cause of hemorrhagic colitis, and no effective therapy exists for E. coli O157:H7 infection. Biofilm formation is closely related to E. coli O157:H7 infection and constitutes a mechanism of antimicrobial resistance. Hence, the antibiofilm or antivirulence approach provides an alternative to antibiotic strategies. Coumarin and its derivatives have a broad range of biological effects, and in this study, the antibiofilm activities of nine coumarins were investigated against E. coli O157:H7. Coumarin or umbelliferone at 50 mu g/ml was found to inhibit biofilm E. coli O157:H7 formation by more than 80% without affecting bacterial growth. Transcriptional analysis showed that coumarins repressed curli genes and motility genes in E. coli O157:H7, and these findings were in-line with observed reductions in fimbriae production, swarming motility, and biofilm formation. In addition, esculetin repressed Shiga-like toxin gene stx2 in E. coli O157:H7 and attenuated its virulence in vivo in the nematode Caenorhabditis elegans. These findings show that coumarins have potential use in antivirulence strategies against persistent E. coli O157:H7 infection. (C) 2014 Elsevier GmbH. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31596http://dx.doi.org/10.1016/j.phymed.2014.04.008
ISSN
0944-7113
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공과대학 > 화학공학부 > Articles
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