Oxygen-dependent enhancement of hydrogen production by engineering bacterial hemoglobin in Escherichia coli

Title
Oxygen-dependent enhancement of hydrogen production by engineering bacterial hemoglobin in Escherichia coli
Author(s)
서정현조병훈[조병훈]김영환[김영환]차형준[차형준]
Keywords
VITREOSCILLA-HEMOGLOBIN; INTRACELLULAR EXPRESSION; FORMATE DEHYDROGENASE; PROTEIN-PRODUCTION; ENERGY-METABOLISM; BIOHYDROGEN; COEXPRESSION; IMPROVEMENT; ALTERS; FLOW
Issue Date
201407
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.39, no.20, pp.10426 - 10433
Abstract
H-2 production under aerobic conditions has been proposed as an alternative method to overcome the fundamentally low yield of H-2 production by fermentative bacteria by maximizing the number of electrons that are available for H-2. Here, we engineered Vitreoscilla hemoglobin (VHb) in Escherichia cob to study the effects of this versatile oxygen (O-2)-binding protein on oxic H-2 production in a closed batch system that was supplemented with glucose. The H-2 yields that were obtained with the VHb-expressing E. coli were greatly enhanced in comparison to the negative control cells in culture that started with high O-2 tensions. The formate hydrogen lyase (FHL) activity of oxically cultured, VHb-expressing cells was also much higher than that of the negative control cells. Through inhibitor studies and time-course experiments, VHb was shown to contribute to the improved H-2 yield primarily by increasing the efficiency of cellular metabolism during the aerobic phase before the onset of H-2 production and not by working as an O-2-scavenger during H-2 production. This new approach allowed more substrate to remain to be further utilized for the production of more H-2 from limited resources. We expect that VHb can be successfully engineered in potential aerobic H-2-producing microbial systems to enhance the overall H-2 production yield. In addition, the remarkably high FHL activity of oxically grown, VHb-expressing cells may make this engineered strain an attractive whole-cell biocatalyst for converting formate to H-2. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved,
URI
http://hdl.handle.net/YU.REPOSITORY/31522http://dx.doi.org/10.1016/j.ijhydene.2014.04.209
ISSN
0360-3199
Appears in Collections:
공과대학 > 화학공학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE