Characterization of a novel composite scaffold consisting of acellular bladder submucosa matrix, polycaprolactone and Pluronic F127 as a substance for bladder reconstruction

Title
Characterization of a novel composite scaffold consisting of acellular bladder submucosa matrix, polycaprolactone and Pluronic F127 as a substance for bladder reconstruction
Author(s)
송필현장유진[장유진]전소영[전소영]김건남[김건남]김진래[김진래]오세형[오세형]김진호[김진호]김범수[김범수]유은상[유은상]권태균[권태균]
Keywords
SMOOTH-MUSCLE; IN-VITRO; POLY(L-LACTIC ACID); CELL-ADHESION; TISSUE; COLLAGEN; REGENERATION; FABRICATION; MORPHOLOGY; MEMBRANE
Issue Date
201407
Publisher
ELSEVIER SCI LTD
Citation
ACTA BIOMATERIALIA, v.10, no.7, pp.3117 - 3125
Abstract
The bladder is an organ susceptible to a variety of congenital anomalies, injuries and disorders. To address the clinical limitations of existing scaffolds, we fabricated a novel scaffold that can be applied to morphological and functional bladder reconstruction. As a first step to prove the benefit of the scaffold, intensive in vitro and in vivo analyses were conducted. The novel composite scaffold was fabricated using polycaprolactone/Pluronic F127 (PCL/F127) and variable proportions (1, 3, 5 and 10 wt.%) of porcine acellular bladder submucosa matrix (BSM). Physicochemical properties and biocompatibilities of the scaffolds were characterized. For cell-mediated analysis, upper-urinary-tract-derived urine stem cells were used. Observations of tensile strength, modulus, porosity, cell adhesion, viability and proliferation characteristics of scaffolds indicated that the optimum proportion of BSM in the composite scaffolds was 3 or 5 wt.%. Based on comparison of 3 and 5 wt.% BSM/PCL/F127 scaffolds with respect to degradability, hydrophilicity, surface properties and functional group presence, the 3 wt.% BSM was chosen for in vivo studies. 8 weeks after kidney-subcapsular implantation of the 3 wt.% BSM/PCL/F127 scaffold, cells remained attached to the surface and there was no evidence of teratomas. A BSM content of 3 wt.% was the optimum proportion for fabrication of the neo scaffold. We predict that the 3 wt.% BSM/PCL/F127 composite scaffold could act as an ideal matrix after cystectomy based on its favorable physicochemical properties and biocompatibilities. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31505http://dx.doi.org/10.1016/j.actbio.2014.03.002
ISSN
1742-7061
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의과대학 > 비뇨기과학교실 > Articles
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