De novo transcriptome assembly of Vitis flexuosa grapevines inoculated with Elsinoe ampelina

Title
De novo transcriptome assembly of Vitis flexuosa grapevines inoculated with Elsinoe ampelina
Author(s)
윤해근안순영김선애조성환[조성환]
Keywords
VINIFERA; DISCOVERY
Issue Date
201407
Publisher
CAMBRIDGE UNIV PRESS
Citation
PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION, v.12, pp.S130 - S133
Abstract
In this study, the transcriptome of Vitis flexuosa leaves inoculated with Elsinoe ampelina was analysed to identify useful genes and elucidate their function and differential expression patterns through assembly and annotation gene ontology of data from sequencing short reads on the Illumina platform. We assembled similar to 121 million high-quality trimmed reads using Velvet and Oases with optimal parameters into a non-redundant set of 70,899 transcripts (>= 200 bp in length). The transcripts exhibited an average length of 1138 bp and a N50 length of 1695 bp, with the largest contig length being 9623 bp. Functional categorization revealed the conservation of genes involved in various molecular functions, including protein binding (21.1%) and oxidoreductase activity (11.7%), in V. flexuosa. The V. flexuosa transcript set generated in this study will serve as a resource for gene discovery and development of functional molecular markers.
URI
http://hdl.handle.net/YU.REPOSITORY/31500http://dx.doi.org/10.1017/S1479262114000410
ISSN
1479-2621
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자연자원대학 > 원예생명과학과 > Articles
자연자원대학 > 원예학과 > Articles
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