Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kappa B and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1

Title
Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kappa B and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1
Author(s)
정태천최재호[최재호]진순우[진순우]한은희[한은희]박봉환[박봉환]김형균[김형균]T Khanal[T Khanal]황용필[황용필]MT Do[MT Do]이현선[이현선]정영철[정영철]김희석[김희석]정혜광[정혜광]
Keywords
NC/NGA MICE; CELLS; RADIX; RATS; INFLAMMATION; INHIBITION; DISEASES; SERUM
Issue Date
201407
Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Citation
PHYTOMEDICINE, v.21, no.8-9, pp.1053 - 1061
Abstract
Purpose: The consequences of precipitously rising allergic skin inflammation rates worldwide have accelerated the risk of atopic dermatitis (AD). Natural product-based agents with good efficacy and low risk of side effects offer promising prevention and treatment strategies for inflammation-related diseases. We have already reported that Platycodon grandiflorum root-derived saponins (Changkil saponins, CKS) have many pharmacological effects, including anti-inflammatory and anti-allergic effects, but its influence on AD remains unclear. Therefore, we evaluated the inhibitory effect of CKS, mainly platycodin D, on AD-like skin symptoms in mice and the possible mechanisms in cells. Methods: Mice were sensitized and challenged with 2,4-dinitrochlorobenzene (DNCB). Four weeks after challenge, mice were treated with oral administration of CKS for 4 weeks. In addition, cells were used to evaluate the effect of CKS, mainly platycodin D, on the TARC expression regulated mechanism. Results: CKS attenuated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-alpha, IFN-gamma, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells and mast cells in the ears. Moreover, CKS and platycodin D inhibited TNF-alpha/IFN-gamma-induced TARC expression through the suppression of NF-kappa B and STAT1 and induction of Nrf2/ARE-mediated hemeoxygenase-1 (HO-1) expression in cells. Conclusion: We suggest that CKS and platycodin D inhibited the development of AD-like skin symptoms by regulating cytokine mediators and may be an effective alternative therapy for AD-like skin symptoms. (C) 2014 Elsevier GmbH. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/31466http://dx.doi.org/10.1016/j.phymed.2014.04.011
ISSN
0944-7113
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약학대학 > 약학부 > Articles
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