Elaidic acid (EA) generates dysfunctional high-density lipoproteins and consumption of EA exacerbates hyperlipidemia and fatty liver change in zebrafish

Title
Elaidic acid (EA) generates dysfunctional high-density lipoproteins and consumption of EA exacerbates hyperlipidemia and fatty liver change in zebrafish
Author(s)
조경현박기훈김종민
Keywords
APOLIPOPROTEIN-A-I; CHOLESTERYL ESTER TRANSFER; TRANSFER PROTEIN-ACTIVITY; HEART-DISEASE; LIPID-FREE; TRANS; SENESCENCE; HDL; METAANALYSIS; MEMBRANE
Issue Date
201407
Publisher
WILEY-BLACKWELL
Citation
MOLECULAR NUTRITION & FOOD RESEARCH, v.58, no.7, pp.1537 - 1545
Abstract
Scope: It is well known that trans-fatty acids have proatherogenic properties while HDL has antiatherogenic activities in plasma. However, there has been no report on the effects of trans-fat on the functional and structural properties of HDL. Methods and results: To compare physiological properties, we synthesized reconstituted HDL (rHDL) containing stearic acid (18:0), oleic acid (18:1, cis), or elaidic acid (EA, 18:1, trans). An rHDL containing EA (EA-rHDL) showed loss of antioxidant ability and induced the highest uptake of oxidized LDL into human macrophages. EA-rHDL caused the strongest cellular senescence in human dermal fibroblast cells along with the highest production of inflammatory species in macrophages co-treated with fructose. Injection of EA-rHDL into zebrafish embryos resulted in acute embryonic toxicity with the lowest survivability. Consumption of trans-fat for 20 weeks resulted in remarkable hyperlipidemia, elevation of serum cholesteryl ester transfer protein activity, hepatic inflammation, and fatty liver changes. Conclusion: Incorporation of EA impaired the beneficial effects of rHDL against atherogenesis. In zebrafish, EA-rHDL resulted in acute embryonic toxicity, and consumption of EA caused remarkable hyperlipidemia, inflammation, and fatty liver changes.
URI
http://hdl.handle.net/YU.REPOSITORY/31456http://dx.doi.org/10.1002/mnfr.201300955
ISSN
1613-4125
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생명공학부 > 생명공학부 > Articles
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