Quantitative Determination of Compounds from Akebia quinata by High-Performance Liquid Chromatography

Title
Quantitative Determination of Compounds from Akebia quinata by High-Performance Liquid Chromatography
Author(s)
손종근Nguyen Thi Yen[Nguyen Thi Yen]Nguyen Van Thu[Nguyen Van Thu]Bing Tian Zhao[Bing Tian Zhao]이재현[이재현]김정아[김정아]최재수[최재수]우은란[우은란]우미희[우미희]민병선[민병선]
Keywords
CHLOROGENIC ACID; TRITERPENOID SAPONINS
Issue Date
201407
Publisher
WILEY-V C H VERLAG GMBH
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.35, no.7, pp.1956 - 1964
Abstract
To provide the scientific corroboration of the traditional uses of Akebia quinata (Thunb.) Decne., a detailed analytical examination of A. quinata stems was carried out using a reversed-phase high performance liquid chromatography (RP-HPLC) method coupled to photodiode array detector (PDA) for the simultaneous determination of four phenolic substances; cuneataside D (1), 2-(3,4-dihydroxyphenyl)ethyl-O-beta-D-glucopyranoside (2), 3-caffeoylquinic acid (3) and calceolarioside B (4). Particular attention was focused on the main compound, 3-caffeoylquinic acid (3), which has a range of biological functions. In addition, 2-(3,4-dihydroxyphenyl)ethyl-O-beta-D-glucopyranoside (2) was considered as a discernible marker of A. quinata from its easy confuse plants. The contents of compounds 2 and 3 ranged from 0.72 to 2.68 mg/g and from 1.66 to 5.64 mg/g, respectively. The validation data indicated that this HPLC/PDA assay was used successfully to quantify the four phenolic compounds in A. quinata from different locations using relatively simple conditions and procedures. The pattern-recognition analysis data from 53 samples classified them into two groups, allowing discrimination between A. quinata and comparable herbs. The results suggest that the established HPLC/PDA method is suitable for quantitation and pattern-recognition analyses for a quality evaluation of this medicinal herb.
URI
http://hdl.handle.net/YU.REPOSITORY/31425http://dx.doi.org/10.5012/bkcs.2014.35.7.1956
ISSN
0253-2964
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약학대학 > 약학부 > Articles
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